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Titolo:
Electropolymerization of pyrrole and phenylenediamine over an organic conducting salt based amperometric sensor of increased selectivity for glucose determination
Autore:
Vidal, JC; Mendez, S; Castillo, JR;
Indirizzi:
Univ Zaragoza, Fac Sci, Dept Analyt Chem, Anal Spect & Sensors Grp, E-50009 Univ Zaragoza Zaragoza Spain E-50009 , Anal Spect & Sensors Grp, E-50009
Titolo Testata:
ANALYTICA CHIMICA ACTA
fascicolo: 1-3, volume: 385, anno: 1999,
pagine: 203 - 211
SICI:
0003-2670(19990405)385:1-3<203:EOPAPO>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
CARBON-PASTE ELECTRODE; COLUMN LIQUID-CHROMATOGRAPHY; D-LACTATE DEHYDROGENASE; LACTIC ACID ANALYSIS; ELECTROCHEMICAL IMMOBILIZATION; BIOSENSOR; OXIDASE; POLYMER; TETRATHIAFULVALENE; CONSTRUCTION;
Keywords:
organic conducting salt; electropolymerization; glucose biosensor; polypyrrole; poly(o-phenylenediamine);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Vidal, JC Univ Zaragoza, Fac Sci, Dept Analyt Chem, Anal Spect & Sensors Grp, E-50009 Univ Zaragoza Zaragoza Spain E-50009 ct & Sensors Grp, E-50009
Citazione:
J.C. Vidal et al., "Electropolymerization of pyrrole and phenylenediamine over an organic conducting salt based amperometric sensor of increased selectivity for glucose determination", ANALYT CHIM, 385(1-3), 1999, pp. 203-211

Abstract

Flow injection methodology was used to electropolymerize pyrrole and o-phenylenediamine over an electrode substrate consisting of the organic conducting salt (OCS) tetrathiafulvalene-p-tetracyanoquinodimethane (TTF-TCNQ), using a cyclic scan of potentials from +100 to +650 mV vs Ag/AgCl down arrow. The enzyme glucose oxidase (GOx) was previously entrapped and cross-linkedwith glutaraldehyde within the OCS in order to construct a glucose enzyme sensor. The sensitivity and dynamic features of enzyme biosensors coated with films of polypyrrole (OCS-GOx/PPy) and poly(o-phenylenediamine) (OCS-GOx/oPPD) strongly adhered to the sensor surface, as well as those of a sensorcoated with a bilayer film (OCS-GOx/PPy/oPPD) were examined. The TTF-TCNQ salt efficiently re-oxidizes reduced sites in flavin adenine dinucleotides,thereby allowing the sensor to operate at low potentials. The selectivity against the electroactive interferents ascorbic acid (AA) and uric acid (UA) is substantially improved relative to bare electrodes as a result of the molecular exclusion properties of PPy and oPPD in both the monolayer and bilayer coated sensors. The OCS-GOx/PPy monolayer sensor was used for the enzymatic determination of glucose in a synthetic serum sample. The proposed enzyme biosensors are highly stable and reproducible; they retain over 80% of their initial enzymatic activity for 8-12 days, after daily use in a flowinjection analysis system, after a total of 350-600 substrate determinations had been carried out. (C) 1999 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 21:43:44