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Titolo:
COOH-terminally extended secretins are potent stimulants of pancreatic secretion
Autore:
Solomon, TE; Walsh, JH; Bussjaeger, L; Zong, YM; Hamilton, JW; Ho, FJ; Lee, TD; Reeve, JR;
Indirizzi:
WALos Angeles Vet Affairs Med Ctr, CURE, Digest Dis Res Ctr, Los Angeles, C W Los Angeles Vet Affairs Med Ctr Los Angeles CA USA 90073 Los Angeles, C Univ Calif Los Angeles, Sch Med, Div Digest Dis, Los Angeles, CA 90024 USAUniv Calif Los Angeles Los Angeles CA USA 90024 Los Angeles, CA 90024 USA Kansas City Dept Vet Affairs Med Ctr, Kansas City, MO 64128 USA Kansas City Dept Vet Affairs Med Ctr Kansas City MO USA 64128 O 64128 USA Univ Kansas, Med Ctr, Dept Biochem, Kansas City, KS 66160 USA Univ KansasKansas City KS USA 66160 t Biochem, Kansas City, KS 66160 USA City Hope Res Inst, Beckman Res Inst, Div Immunol, Duarte, CA 91010 USA City Hope Res Inst Duarte CA USA 91010 Div Immunol, Duarte, CA 91010 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
fascicolo: 4, volume: 39, anno: 1999,
pagine: G808 - G816
SICI:
0193-1857(199904)39:4<G808:CESAPS>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
MOLECULAR-CLONING; PLASMA SECRETIN; FUNCTIONAL EXPRESSION; ADENYLATE-CYCLASE; PORCINE SECRETIN; SODIUM OLEATE; RAT SECRETIN; FORM; RECEPTOR; BIOACTIVITY;
Keywords:
posttranslational processing; amidation; radioimmunoassay; physiology;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Solomon, TE W Los Angeles Vet Affairs Med Ctr, CURE, Digest Dis Res Ctr, 11301 Wilshire W Los Angeles Vet Affairs Med Ctr 11301 Wilshire Blvd,Bldg 115,Rm 111 Los Angeles CA USA 90073
Citazione:
T.E. Solomon et al., "COOH-terminally extended secretins are potent stimulants of pancreatic secretion", AM J P-GAST, 39(4), 1999, pp. G808-G816

Abstract

Posttranslational processing of preprosecretin generates several COOH-terminally extended forms of secretin and alpha-carboxyl amidated secretin. We used synthetic canine secretin analogs with COOH-terminal -amide,-Gly, or -Gly-Lys-Arg to examine the effects of COOH-terminal extensions of secretin on bioactivity and detection in RIA Synthetic products were purified by reverse-phase and ion-exchange HPLC and characterized by reverse-phase isocratic HPLC and amino acid, sequence, and mass spectral analyses. Secretin and secretin-Gly were noted to coelute during reverse-phase HPLC. In RIA using eight, different antisera raised against amidated secretin, COOH-terminallyextended secretins had little or no cross-reactivity. Bioactivity was assessed by measuring pancreatic responses in anesthetized rats. Amidated canine and porcine secretins were equipotent. Secretin-Gly and secretin-Gly-Lys-Arg had potencies of 81 +/- 9% (P > 0.05) and 176 +/- 13% (P < 0.01), respectively, compared with amidated secretin, and the response to secretin-Gly-Lys-Arg lasted significantly longer These data demonstrate that 1) amidatedsecretin and secretin;Gly are not separable under some chromatographic conditions, 2) current RIA may not detect bioactive COOH-terminally extended forms of secretin in tissue extracts or blood, and 3) the secretin receptor mediating stimulation of pancreatic secretion recognizes both amidated and COOH-terminally extended secretins.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 02:00:07