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Titolo:
Linker for activation of T cells (LAT), a novel immunohistochemical markerfor T cells, NK cells, mast cells, and megakaryocytes - Evaluation in normal and pathological conditions
Autore:
Facchetti, F; Chan, JKC; Zhang, WG; Tironi, A; Chilosi, M; Parolini, S; Notarangelo, LD; Samelson, LE;
Indirizzi:
Univ Brescia, Dept Pathol, Spedali Civili Brescia, I-25124 Brescia, Italy Univ Brescia Brescia Italy I-25124 ivili Brescia, I-25124 Brescia, Italy Univ Brescia, Dept Biomed Sci & Biotechnol, Brescia, Italy Univ Brescia Brescia Italy Dept Biomed Sci & Biotechnol, Brescia, Italy Univ Brescia, Dept Pediat, Brescia, Italy Univ Brescia Brescia ItalyUniv Brescia, Dept Pediat, Brescia, Italy Queen Elizabeth Hosp, Hong Kong, Hong Kong Queen Elizabeth Hosp Hong Kong Hong Kong eth Hosp, Hong Kong, Hong Kong Univ Verona, I-37100 Verona, Italy Univ Verona Verona Italy I-37100Univ Verona, I-37100 Verona, Italy NIH,2Sect Lymphocyte Signaling, Cell Biol & Metab Branch, Bethesda, MD 2089 NIH Bethesda MD USA 20892 ng, Cell Biol & Metab Branch, Bethesda, MD 2089
Titolo Testata:
AMERICAN JOURNAL OF PATHOLOGY
fascicolo: 4, volume: 154, anno: 1999,
pagine: 1037 - 1046
SICI:
0002-9440(199904)154:4<1037:LFAOTC>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
PARAFFIN-EMBEDDED TISSUE; NATURAL-KILLER-CELLS; TERMINAL DEOXYNUCLEOTIDYL TRANSFERASE; NON-HODGKINS-LYMPHOMA; TYROSINE PHOSPHORYLATION; B-CELL; ADAPTER PROTEIN; MICE LACKING; CD3 ANTIGEN; RECEPTOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Facchetti, F Univ Brescia, Dept Pathol, Spedali Civili Brescia, I-25124 Brescia, Italy Univ Brescia Brescia Italy I-25124 , I-25124 Brescia, Italy
Citazione:
F. Facchetti et al., "Linker for activation of T cells (LAT), a novel immunohistochemical markerfor T cells, NK cells, mast cells, and megakaryocytes - Evaluation in normal and pathological conditions", AM J PATH, 154(4), 1999, pp. 1037-1046

Abstract

LAT (linker for activation of T cells) is an integral membrane protein of 36-38 kd that plays an important role in T cell activation. Using a rabbit polyclonal antibody generated against the cytosolic portion of LAT, we investigated the immunohistochemical expression of LAT in normal and pathological hematolymphoid tissues. LAT reacts with human T cells in paraffin sections, including decalcified bone marrow trephines, LAT appears early in T cells at the thymocyte stage and before TdT expression in embryos, and is expressed in peripheral lymphoid tissues, without restriction to any T cell subpopulations. in addition to T cells, natural killer (NK) cells (evaluated with now cytometry), megakaryocytes and mast cells are also LAT-positive, whereas B cells and other myeloid and monocytic derived cells are negative. Tested on a total of 264 paraffin-embedded tissue biopsies, LET reacted withthe great majority (96.8%) of T/NK-cell neoplasms, covering the full rangeof T cell maturation. Although antibodies to both LAT and CD3 had a similarly high sensitivity in the staining of T/NK-cell lymphomas, when used in conjunction, they successfully identified a higher number of cases (98.4%). Atypical megakaryocytes from different hematological disorders, as well as mast cells in mastocytosis were also LAT-positive, but all neoplasms of B cell origin, Hodgkin's lymphomas, and several nonlymphoid malignancies were negative. These data indicate that the anti-LAT antibody may be of value todiagnostic histopathologists for the identification of T cell neoplasms.

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Documento generato il 27/10/20 alle ore 05:29:44