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Titolo:
LITHIUM HOMEOSTASIS IN XENOPUS OOCYTES - IMPLICATIONS FOR THE STUDY OF SIGNAL-TRANSDUCTION
Autore:
GOMEZ JR; KARKANIAS NB; LENOX RH; PAPKE RL;
Indirizzi:
UNIV FLORIDA,COLL MED,JH MILLER HLTH CTR,DEPT PHARMACOL,BOX 100267 GAINESVILLE FL 32610 UNIV FLORIDA,COLL MED,JH MILLER HLTH CTR,DEPT PHARMACOL GAINESVILLE FL 32610 UNIV FLORIDA,COLL MED,JH MILLER HLTH CTR,DEPT THERAPEUT GAINESVILLE FL 32610 UNIV FLORIDA,COLL MED,JH MILLER HLTH CTR,DEPT NEUROSCI GAINESVILLE FL32610
Titolo Testata:
Life sciences (1973)
fascicolo: 19, volume: 63, anno: 1998,
pagine: 1715 - 1724
SICI:
0024-3205(1998)63:19<1715:LHIXO->2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
INHIBITORS;
Keywords:
LITHIUM; HOMEOSTASIS; XENOPUS OOCYTES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
15
Recensione:
Indirizzi per estratti:
Citazione:
J.R. Gomez et al., "LITHIUM HOMEOSTASIS IN XENOPUS OOCYTES - IMPLICATIONS FOR THE STUDY OF SIGNAL-TRANSDUCTION", Life sciences (1973), 63(19), 1998, pp. 1715-1724

Abstract

The Xenopus oocyte has been shown to be a useful model for the study of signal transduction pathways. The present study investigated whether or not the oocyte could be used to study the effects of lithium on signal transduction mechanisms by comparing the dynamics of lithium homeostasis in the oocyte and a human immortalized hippocampal cell line using Flame Atomic Emission Spectroscopy (FAES). A biphasic pattern oflithium uptake was observed in the oocyte in the presence of 5 mM extracellular lithium. The late phase of lithium uptake, which started after 30 minutes of incubation time, was sensitive to phloretin, an inhibitor of Na+/Li+ counter-transport. Differences in lithium efflux kinetics further characterized the two observed phases of accumulation andalso suggested that lithium might be distributed in different pools within the oocyte, including one sequestered in organelles or associated with cytosolic proteins. An analogous sequestered pool was not, however, observed in the hippocampal cell line indicating that lithium is distributed differently in these cell types. This suggests that the Xenopus oocyte might not be a suitable model for evaluating the effects of lithium on signal transduction pathways because of the unknown contribution of the sequestered pool on predicting relevant physiological effects.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 23:31:09