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Titolo:
DEVELOPMENT OF CALCIUM-PERMEABLE LPHA-AMINO-3-HYDROXY-5-METHYL-4-ISOXAZOLEPROPIONIC ACID RECEPTORS IN CULTURED NEOCORTICAL NEURONS VISUALIZED BY COBALT STAINING
Autore:
JENSEN JB; SCHOUSBOE A; PICKERING DS;
Indirizzi:
ROYAL DANISH SCH PHARM,DEPT PHARMACOL,PHARMABIOTEC RES CTR,2 UNIV PK DK-2100 COPENHAGEN DENMARK ROYAL DANISH SCH PHARM,DEPT PHARMACOL,PHARMABIOTEC RES CTR DK-2100 COPENHAGEN DENMARK
Titolo Testata:
Journal of neuroscience research
fascicolo: 2, volume: 54, anno: 1998,
pagine: 273 - 281
SICI:
0360-4012(1998)54:2<273:DOCL>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXCITATORY AMINO-ACIDS; CENTRAL NERVOUS-SYSTEM; METHYL-D-ASPARTATE; GLUTAMATE RECEPTORS; HIPPOCAMPAL-NEURONS; AMPA/KA RECEPTORS; KAINATE RECEPTORS; GLIAL-CELLS; CA2+ INFLUX; IN-VITRO;
Keywords:
KAINATE RECEPTORS; DESENSITIZATION; CYCLOTHIAZIDE; CONCANAVALIN A; GYKI 53655;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
37
Recensione:
Indirizzi per estratti:
Citazione:
J.B. Jensen et al., "DEVELOPMENT OF CALCIUM-PERMEABLE LPHA-AMINO-3-HYDROXY-5-METHYL-4-ISOXAZOLEPROPIONIC ACID RECEPTORS IN CULTURED NEOCORTICAL NEURONS VISUALIZED BY COBALT STAINING", Journal of neuroscience research, 54(2), 1998, pp. 273-281

Abstract

The developmental expression of calcium (Ca2+)permeable lpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) and kainate receptors in cultured neocortical neurons was evaluated by using cobalt uptake, a histochemical method that identifies cells expressing Ca2+-permeable, non-N-methyl-D-aspartate (non-NMDA) receptors. At a concentration of 500 mu M, AMPA was found to stimulate cobalt uptake only late in development, resulting in staining of 2.7% +/- 0.3% of the neurons maintained in culture for 12 days in vitro (DIV). When AMPA receptor desensitization was blocked with 50 mu M cyclothiazide, the developmental profile of cobalt uptake mediated by 25 mu M AR;IPA changed dramatically. The cobalt staining now appeared in young cultures (5 DIV), and the percentage of stained cells increased from 3.4% +/- 0.2% at 5 DIV to 21.7% +/- 1.6% at 12 DIV. The effect of 200 mu M kainate was similar tothat seen with 25 mu M AMPA plus 50 mu M cyclothiazide, resulting in 17.7% +/- 0.3% stained neurons at 12 DIV. The cobalt uptake was specific to AMPA and kainate receptors because NMDA receptors and voltage-gated calcium channels were found not to mediate any cobalt staining. Inaddition, 10 mu M 6-nitro-7-sulphamoylbenzo[f]-quinoxaline-2,3-dione (NBQX) was able to prevent all staining at 5 and 8 DIV and most of thestaining at 12 DIV, indicating that the non-NMDA ionotropic glutamatereceptors are involved in cobalt uptake into the neurons. The AMPA receptor-selective antagonist GYKI 53655 was used to differentiate between cobalt influx through AMPA- or kainate-preferring receptors. After pretreatment with concanavalin A (con A), an inhibitor of kainate receptor desensitization, cobalt uptake was assessed after stimulation by 200 mu M kainate in the presence of 25 mu M GYKI 53655. No cobalt staining was observed under these conditions, indicating that most if not all of the cobalt influx induced by kainate was mediated through AMPA receptor channels. (C) 1998 Wiley-Liss,Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 04:33:07