Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
THE TERNARY MICROPLASMIN-STAPHYLOKINASE-MICROPLASMIN COMPLEX IS A PROTEINASE-COFACTOR-SUBSTRATE COMPLEX IN ACTION
Autore:
PARRY MAA; FERNANDEZCATALAN C; BERGNER A; HUBER R; HOPFNER KP; SCHLOTT B; GUHRS KH; BODE W;
Indirizzi:
MAX PLANCK INST BIOCHEM,DEPT STRESS RES,KLOPFERSPITZ 18A D-82152 MARTINSRIED GERMANY INST MOL BIOTECHNOL JENA GERMANY
Titolo Testata:
Nature structural biology
fascicolo: 10, volume: 5, anno: 1998,
pagine: 917 - 923
SICI:
1072-8368(1998)5:10<917:TTMCIA>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLASMINOGEN-ACTIVATOR; CRYSTAL-STRUCTURE; BLOOD-COAGULATION; CATALYTIC DOMAIN; FACTOR IXA; SITE; MUTAGENESIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
M.A.A. Parry et al., "THE TERNARY MICROPLASMIN-STAPHYLOKINASE-MICROPLASMIN COMPLEX IS A PROTEINASE-COFACTOR-SUBSTRATE COMPLEX IN ACTION", Nature structural biology, 5(10), 1998, pp. 917-923

Abstract

The serine proteinase plasmin is the key fibrinolytic enzyme that dissolves blood clots and also promotes cell migration and tissue remodeling. Here, we report the 2.65 Angstrom crystal structure of a ternary complex of microplasmin-staphylokinase bound to a second microplasmin. The staphylokinase 'cofactor' does not affect the active-site geometry of the plasmin 'enzyme', but instead modifies its subsite specificity by providing additional docking sites for enhanced presentation of the plasminogen 'substrate' to the 'enzymes's' active site. The activation loop of the plasmin 'substrate', cleaved in these crystals, can bereconstructed to show how it runs across the active site of the plasmin 'enzyme' prior to activation cleavage. This is the first experimental structure of a productive proteinase-cofactor-macromolecular substrate complex. Furthermore, it provides a template for the design of improved plasminogen activators and plasmin inhibitors with considerable therapeutical potential.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 18:48:17