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Titolo:
A NOVEL ATP-BINDING CASSETTE TRANSPORTER INVOLVED IN MULTIDRUG-RESISTANCE IN THE PHYTOPATHOGENIC FUNGUS PENICILLIUM-DIGITATUM
Autore:
NAKAUNE R; ADACHI K; NAWATA O; TOMIYAMA M; AKUTSU K; HIBI T;
Indirizzi:
UNIV TOKYO,DEPT AGR & ENVIRONM BIOL,BUNKYO KU TOKYO 1138657 JAPAN UNIV TOKYO,DEPT AGR & ENVIRONM BIOL,BUNKYO KU TOKYO 1138657 JAPAN IBARAKI UNIV,FAC AGR AMI IBARAKI 3000393 JAPAN NATL INST AGROBIOL RESOURCES,DEPT BIOTECHNOL TSUKUBA IBARAKI 3058602 JAPAN
Titolo Testata:
Applied and environmental microbiology (Print)
fascicolo: 10, volume: 64, anno: 1998,
pagine: 3983 - 3988
SICI:
0099-2240(1998)64:10<3983:ANACTI>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
EBURICOL 14-ALPHA-DEMETHYLASE CYP51; SACCHAROMYCES-CEREVISIAE; CANDIDA-ALBICANS; ASPERGILLUS-NIDULANS; MOLECULAR-CLONING; ENCODING GENE; WILD-TYPE; YEAST; PDR5; ACCUMULATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
44
Recensione:
Indirizzi per estratti:
Citazione:
R. Nakaune et al., "A NOVEL ATP-BINDING CASSETTE TRANSPORTER INVOLVED IN MULTIDRUG-RESISTANCE IN THE PHYTOPATHOGENIC FUNGUS PENICILLIUM-DIGITATUM", Applied and environmental microbiology (Print), 64(10), 1998, pp. 3983-3988

Abstract

Demethylation inhibitor (DMI)-resistant strains of the plant pathogenic fungus Penicillium digitatum were shown to be simultaneously resistant to cycloheximide, 4-nitroquinoline-N-oxide (4NQO), and acriflavine. A PMR1 (Penicillium multidrug resistance) gene encoding an ATP binding cassette (ABC) transporter (P-glycoprotein) was cloned from a genomic DNA library of a DMI-resistant strain (LC2) of Penicillium digitatum by heterologous hybridization with a DNA fragment containing an ABC-encoding region from Botrytis cinerea. Sequence analysis revealed significant amino acid homology to the primary structures of PMR1 (proteinencoded by the PMR1 gene) and ABC transporters of Saccharomyces cerevisiae (PDR5 and SNQ2), Schizosaccharomyces pombe (HBA2), Candida albicans (CDR1), and Aspergillus nidulans (AtrA and AtrB). Disruption of the PMR1 gene of P. digitatum DMI-resistant strain LC2 demonstrated thatPMR1 was an important determinant of resistance to DMIs. The effective concentrations inhibiting radial growth by 50% (EC(50)s) and the MICs of fenarimol and bitertanol for the PMR1 disruptants (Delta pmr1 mutants) were equivalent to those for DMI-sensitive strains. Northern blot analysis indicated that severalfold more PMR1 transcript accumulatedin the DMI-resistant strains compared with those in DMI-sensitive strains in the absence of fungicide. In both DMI-resistant and -sensitivestrains, transcription of PMR1 was strongly enhanced within 10 min after treatment,vith the DMI fungicide triflumizole. These results suggested that the toxicant efflux system comprised of PMR1 participates directly in the DMI resistance of the fungus.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 07:37:45