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Titolo:
CROSS-SPECIES AMPLIFICATION OF SOYBEAN (GLYCINE-MAX) SIMPLE SEQUENCE REPEATS (SSRS) WITHIN THE GENUS AND OTHER LEGUME GENERA - IMPLICATIONSFOR THE TRANSFERABILITY OF SSRS IN PLANTS
Autore:
PEAKALL R; GILMORE S; KEYS W; MORGANTE M; RAFALSKI A;
Indirizzi:
AUSTRALIAN NATL UNIV,DIV BOT & ZOOL CANBERRA ACT 0200 AUSTRALIA UNIV UDINE,DIPARTIMENTO PROD VEGETALE & TECNOL AGR I-33100 UDINE ITALY DUPONT CO INC,DUPONT AGR PROD,BIOTECHNOL RES EXPT STN WILMINGTON DE 00000
Titolo Testata:
Molecular biology and evolution
fascicolo: 10, volume: 15, anno: 1998,
pagine: 1275 - 1287
SICI:
0737-4038(1998)15:10<1275:CAOS(S>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
MICROSATELLITE LOCI; SUBGENUS GLYCINE; ARABIDOPSIS-THALIANA; DNA MARKERS; DIRECTIONAL EVOLUTION; LENGTH POLYMORPHISM; ALLELE SIZE; CONSERVATION; IDENTIFICATION; CHIMPANZEES;
Keywords:
GLYCINE; SOYBEAN; MICROSATELLITES; SIMPLE SEQUENCE REPEATS (SSRS); CROSS-SPECIES AMPLIFICATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
67
Recensione:
Indirizzi per estratti:
Citazione:
R. Peakall et al., "CROSS-SPECIES AMPLIFICATION OF SOYBEAN (GLYCINE-MAX) SIMPLE SEQUENCE REPEATS (SSRS) WITHIN THE GENUS AND OTHER LEGUME GENERA - IMPLICATIONSFOR THE TRANSFERABILITY OF SSRS IN PLANTS", Molecular biology and evolution, 15(10), 1998, pp. 1275-1287

Abstract

We investigated the transferability of 31 soybean (Glycine man) simple sequence repeat (SSR) loci to wild congeners and to other legume genera. Up to 65% of the soybean primer pairs amplified SSRs within Glycine, but frequently, the SSRs were short and interrupted compared with those of soybeans. Nevertheless, 85% of the loci were polymorphic within G. clandestina. Cross-species amplification outside of the genus was much lower (3%-13%), with polymorphism restricted to one primer pair, AG81. AG81 amplified loci in Glycine, Kennedia, and Vigna (Phaseoleae), Vicia (Vicieae), Trifolium (Trifolieae), and Lupinus (Genisteae) within the Papilionoideae, and in Albizia within the Mimosoideae. The primer conservation at AG81 may be explained by its apparent proximity to the seryl-tRNA synthetase gene. Interspecific differences in allelesize at AG81 loci reflected repeat length variation within the SSR region and indels in the flanking region. Alleles of identical size withdifferent underlying sequences (size homoplasy) were observed. Our findings and the emerging patterns in other plant studies suggest that in contrast to animals, successful cross-species amplification of SSRs in plants is largely restricted to congeners or closely related genera. Because mutations in both the SSR region and the flanking region contribute to variation in allele size among species, knowledge of DNA sequence is essential before SSR loci can be meaningfully used to address applied and evolutionary questions.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 09:36:58