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Titolo:
ASCORBATE AND GLUTATHIONE HOMEOSTASIS IN VASCULAR SMOOTH-MUSCLE CELLS- COOPERATION WITH ENDOTHELIAL-CELLS
Autore:
VOSKOBOINIK I; SODERHOLM K; COTGREAVE IA;
Indirizzi:
KAROLINSKA INST,INST ENVIRONM MED,DIV BIOCHEM TOXICOL,BOX 210 S-17177STOCKHOLM SWEDEN KAROLINSKA INST,INST ENVIRONM MED,DIV BIOCHEM TOXICOL S-17177 STOCKHOLM SWEDEN
Titolo Testata:
American journal of physiology. Cell physiology
fascicolo: 4, volume: 44, anno: 1998,
pagine: 1031 - 1039
SICI:
0363-6143(1998)44:4<1031:AAGHIV>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTRACELLULAR GLUTATHIONE; IN-VITRO; ACID; PERMEABILITY; EXPRESSION; TRANSPORT; DISEASE; CULTURE; INJURY; STRESS;
Keywords:
HUMAN VASCULAR SMOOTH MUSCLE CELL ANTIOXIDANT REGULATION; ENDOTHELIAL CELL-SMOOTH MUSCLE CELL INTERACTIONS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
29
Recensione:
Indirizzi per estratti:
Citazione:
I. Voskoboinik et al., "ASCORBATE AND GLUTATHIONE HOMEOSTASIS IN VASCULAR SMOOTH-MUSCLE CELLS- COOPERATION WITH ENDOTHELIAL-CELLS", American journal of physiology. Cell physiology, 44(4), 1998, pp. 1031-1039

Abstract

Human umbilical vein smooth muscle cells (HUVSMCs) utilize extracellular cystine, glutathione (GSH), and N-acetylcysteine (NAC) to synthesize cellular GSH. Extracellular cystine was effective from 5 mu M, whereas GSH and NAC were required at 100 mu M for comparable effects. The efficacy of extracellular GSH was dependent on de novo GSH synthesis, indicating a dependence on cellular gamma-glutamyltransferase (glutamyl transpeptidase). Coculture of syngenetic HUVSMCs and corresponding human umbilical vein endothelial cells (HUVECs) on porous supports restricted cystine- or GSH-stimulated synthesis of HUVSMC GSH when supplied on the ''luminal'' endothelial side. Thus HUVSMC GSH rapidly attained a steady-state level below that achieved in the absence of interposed HUVECs. HUVSMCs also readily utilize both reduced ascorbate (AA) andoxidized dehydroascorbate (DHAA) over the range 50-500 mu M. Phloretin effectively blocked both AA- and DHAA-stimulated assimilation of intracellular AA, indicating a role for a glucose transporter in their transport. Uptake of extracellular AA was also sensitive to extracellular, but not intracellular, thiol depletion. When AA was applied to the endothelial side of the coculture model, assimilation of intracellularAA in HUVSMCs was restricted to a steady-state level below that achieved by free access.

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Documento generato il 22/09/20 alle ore 18:18:22