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Titolo:
INDUCTION OF GLUT1 MESSENGER-RNA IN RESPONSE TO AZIDE AND INHIBITION OF PROTEIN-SYNTHESIS
Autore:
BEHROOZ A; ISMAILBEIGI F;
Indirizzi:
CASE WESTERN RESERVE UNIV,DIV CLIN & MOL ENDOCRINOL,DEPT MED,10900 EUCLID AVE CLEVELAND OH 44106 CASE WESTERN RESERVE UNIV,DIV CLIN & MOL ENDOCRINOL,DEPT MED CLEVELAND OH 44106 CASE WESTERN RESERVE UNIV,DEPT MOL BIOL CLEVELAND OH 44106 CASE WESTERN RESERVE UNIV,DEPT MICROBIOL CLEVELAND OH 44106
Titolo Testata:
Molecular and cellular biochemistry
fascicolo: 1-2, volume: 187, anno: 1998,
pagine: 33 - 40
SICI:
0300-8177(1998)187:1-2<33:IOGMIR>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLUCOSE-TRANSPORTER EXPRESSION; MESSENGER-RNA DEGRADATION; 3' UNTRANSLATED REGION; NECROSIS-FACTOR-ALPHA; LIVER CELL-LINE; GENE-EXPRESSION; C-FOS; OXIDATIVE-PHOSPHORYLATION; CLONE-9 CELLS; GROWTH-FACTORS;
Keywords:
GLUT1 MESSENGER-RNA; GLUT1 GENE TRANSCRIPTION; AZIDE; ANISOMYCIN; EMETINE; SAPK; C-JUN KINASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
46
Recensione:
Indirizzi per estratti:
Citazione:
A. Behrooz e F. Ismailbeigi, "INDUCTION OF GLUT1 MESSENGER-RNA IN RESPONSE TO AZIDE AND INHIBITION OF PROTEIN-SYNTHESIS", Molecular and cellular biochemistry, 187(1-2), 1998, pp. 33-40

Abstract

Incubation of Clone 9 cells, a nontransformed rat liver cell line, inthe presence of 5 mM azide results in an induction of GLUT1 mRNA which becomes detectable after similar to 3 h of continuous exposure to the agent. In examining the role of on-going protein synthesis in this response, we found that: (i) the induction of GLUT1 mRNA by azide was not inhibited by anisomycin, (ii) exposure to anisomycin alone also resulted in increased GLUT1 mRNA content, and (iii) the increments in GLUT1 mRNA content in the presence of both azide and anisomycin were additive. Following exposure to 30 mu M anisomycin, the increase in GLUT1 mRNA content became evident at 1 h, reached a maximum level of similarto 7-fold at 3 h, then slowly decreased but remained elevated at similar to 2-fold control levels at 12 h. Transcription of the GLUT 1 gene, estimated by nuclear run-on assay, was stimulated 1.4 +/- 0.1 and 1.6 +/- 0.2-fold in cells exposed to anisomycin for 1 and 2 h, respectively (p < 0.05 for both). Upon inhibition of RNA synthesis by actinomycin D, GLUT1 mRNA content decreased with a half-life of 1.9 +/- 0.4 h in control cells, while in contrast, GLUT 1 mRNA half-life was 4.6 +/- 0.8 h in cells exposed to anisomycin. The induction of GLUT1 mRNA by anisomycin was half-maximal at similar to 3 mu M, whereas inhibition ofleucine incorporation and stimulation of Stress Activated Protein Kinase (SAPK), measured as c-Jun N-terminal kinase activity, were half-maximal at similar to 0.3 and similar to 0.05 mu M anisomycin, respectively. GLUT1 mRNA content was also increased by the protein synthesis inhibitor emetine, and the effect was associated with no stimulation of SAPK activity. Finally, SAPK activity was minimally stimulated in cells exposed to azide. It is concluded that: ( 1) on-going protein synthesis is not necessary for the induction of GLUT1 mRNA content in response to azide, (2) the induction of GLUT 1 mRNA by anisomycin is relatedto its activity to inhibit protein synthesis, and (3) under basal conditions, a rapidly turning-over putative protein exerts a negative regulatory effect on GLUT1 mRNA expression.

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Documento generato il 02/07/20 alle ore 19:03:40