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Titolo:
EXPRESSION OF THE ACTIVATION ANTIGEN CD69 PREDICTS FUNCTIONALITY OF IN-VITRO EXPANDED PERIPHERAL-BLOOD MONONUCLEAR-CELLS (PBMC) FROM HEALTHY DONORS AND HIV-INFECTED PATIENTS
Autore:
NIELSEN SD; AFZELIUS P; ERSBOLL AK; NIELSEN JO; HANSEN JES;
Indirizzi:
HVIDOVRE UNIV HOSP,INFECT DIS LAB,144 DK-2650 HVIDOVRE DENMARK HVIDOVRE UNIV HOSP,INFECT DIS LAB DK-2650 HVIDOVRE DENMARK ROYAL VET & AGR UNIV,DEPT ANIM SCI & ANIM HLTH FREDERIKSBERG DENMARK
Titolo Testata:
Clinical and experimental immunology
fascicolo: 1, volume: 114, anno: 1998,
pagine: 66 - 72
SICI:
0009-9104(1998)114:1<66:EOTAAC>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
FLOW CYTOMETRIC ANALYSIS; CYTOTOXIC T-LYMPHOCYTES; POKEWEED MITOGEN; IN-VITRO; RECEPTOR EXPRESSION; ADOPTIVE TRANSFER; CD4 LYMPHOCYTES; IDENTICAL-TWINS; HOMOSEXUAL MEN; IMMUNODEFICIENCY;
Keywords:
HIV; LYMPHOCYTE EXPANSION; PROLIFERATION; CD69; GENE THERAPY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
S.D. Nielsen et al., "EXPRESSION OF THE ACTIVATION ANTIGEN CD69 PREDICTS FUNCTIONALITY OF IN-VITRO EXPANDED PERIPHERAL-BLOOD MONONUCLEAR-CELLS (PBMC) FROM HEALTHY DONORS AND HIV-INFECTED PATIENTS", Clinical and experimental immunology, 114(1), 1998, pp. 66-72

Abstract

Gene therapy for AIDS necessitates harvest and expansion of PBMC fromHIV-infected patients. We expanded PBMC from healthy blood donors andHIV-infected patients for up to 14 days using four expansion protocols: 3 days of phytohaemagglutinin (PHA) stimulation, continuous PHA stimulation, 3 days of stimulation with anti-CD3 and anti-CD28, and continuous stimulation with anti-CD3 and anti-CD28. Functionality of PBMC was evaluated prior to and after expansion using standard proliferationassay. Phenotype and lymphocyte subset activation defined by expression of CD69 and CD25 were determined using flow cytometry. PBMC from healthy donors and HIV-infected patients were readily expanded. The bestexpansion was obtained using stimulation for 3 days. After expansion,functionality of PBMC measured as proliferative response was partly conserved. PBMC expanded with stimulation for 3 days exhibited more preserved functionality than PBMC stimulated continuously (P < 0.03). Themean proliferative response in each of the four different expansion protocols correlated with the mean values of CD69 expression. The proliferative responses from patients and healthy donors expanded with PHA stimulation for 3 days correlated with CD69 expression on CD4 cells (r= 0.68, P < 0.01) and on CD8 cells (r = 0.59, P < 0.03). Furthermore,expression of CD69 reliably predicted which patients and donors had highly conserved functionality after in vitro expansion. Finally, PBMC expanded with PHA stimulation for 3 days were examined for apoptosis. Only a minor fraction was primed for apoptosis, and this fraction could be significantly reduced by addition of IL-2 to the culture medium (P < 0.05). In conclusion, the feasibility of expanding PBMC from HIV patients was demonstrated. Expanded PBMC had conserved functionality. Finally, after in vitro expansion, expression of the activation antigenCD69 reliably predicted functionality of PBMC.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 04:54:04