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Titolo:
PROTEOGLYCANS MEDIATE CATIONIC LIPOSOME-DNA COMPLEX-BASED GENE DELIVERY IN-VITRO AND IN-VIVO
Autore:
MOUNKES LC; ZHONG W; CIPRESPALACIN G; HEATH TD; DEBS RJ;
Indirizzi:
CALIF PACIFIC MED RES INST,STERN BLDG,2330 CLAY ST SAN FRANCISCO CA 94115 CALIF PACIFIC MED RES INST SAN FRANCISCO CA 94115 UNIV WISCONSIN,SCH PHARM MADISON WI 53706
Titolo Testata:
The Journal of biological chemistry
fascicolo: 40, volume: 273, anno: 1998,
pagine: 26164 - 26170
SICI:
0021-9258(1998)273:40<26164:PMCLCG>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEPARAN-SULFATE PROTEOGLYCANS; RECEPTOR-RELATED PROTEIN; FIBROBLAST GROWTH; CELL-SURFACE; EXPRESSION; MATRIX; DEGRADATION; MECHANISM; RELEASE; BINDING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
46
Recensione:
Indirizzi per estratti:
Citazione:
L.C. Mounkes et al., "PROTEOGLYCANS MEDIATE CATIONIC LIPOSOME-DNA COMPLEX-BASED GENE DELIVERY IN-VITRO AND IN-VIVO", The Journal of biological chemistry, 273(40), 1998, pp. 26164-26170

Abstract

The factors controlling cationic liposome-DNA complex (CLDC)-based gene transfer in cells and in animals are poorly understood. me found that cell surface heparin/heparan sulfate-bearing proteoglycans mediate CLDC-based gene transfer and expression both in cultured cells and following intravenous gene delivery into animals. CLDC did not transfect Raji cells, which lack proteoglycans, but did efficiently transfect Raji cells stably transfected with the proteoglycan, syndecan-1, Fucoidan, heparin, or dextran sulfate, all of which are highly anionic polysaccharides, each blocked CLDC-mediated transfection both in cultured cells and following intravenous injection into mice, but had no effect on transfection by either recombinant adenovirus infection or electroporation. Intravenous pretreatment of mice with heparinases, which specifically cleave heparan sulfate molecules from cell surface proteoglycans, blocked intravenous, CLDC-mediated transfection in mice, confirming that proteoglycans mediate CLDC gene delivery in vivo. Modulation ofproteoglycan expression may prove useful in controlling the efficiency of, as well as targeting the sites of, CLDC-based gene transfer in animals.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/07/20 alle ore 17:55:17