Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
ROLE OF EP2 RECEPTORS AND CAMP IN PROSTAGLANDIN E-2 REGULATED EXPRESSION OF TYPE-I COLLAGEN ALPHA-1, LYSYL OXIDASE, AND CYCLOOXYGENASE-1 GENES IN HUMAN EMBRYO LUNG FIBROBLASTS
Autore:
CHOUNG J; TAYLOR L; THOMAS K; ZHOU X; KAGAN H; YANG X; POLGAR P;
Indirizzi:
BOSTON UNIV,SCH MED,DEPT BIOCHEM BOSTON MA 02118 BOSTON UNIV,SCH MED,DEPT BIOCHEM BOSTON MA 02118
Titolo Testata:
Journal of cellular biochemistry
fascicolo: 2, volume: 71, anno: 1998,
pagine: 254 - 263
SICI:
0730-2312(1998)71:2<254:ROERAC>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROSTANOID RECEPTORS; FUNCTIONAL EXPRESSION; EP(2) SUBTYPE; CELLS; CLONING; BETA; E(2); CHONDROCYTES; ACTIVATION; CDNA;
Keywords:
LYSYL OXIDASE; CYCLOOXYGENASE-1; TYPE I COLLAGEN ALPHA-1; PROSTAGLANDIN E-2; PROSTAGLANDIN E-2 RECEPTORS; CYCLIC AMP; INTERLEUKIN-1-BETA; TRANSFORMING GROWTH FACTOR-BETA; FORSKOLIN; 11-DEOXY PGE(1);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
29
Recensione:
Indirizzi per estratti:
Citazione:
J. Choung et al., "ROLE OF EP2 RECEPTORS AND CAMP IN PROSTAGLANDIN E-2 REGULATED EXPRESSION OF TYPE-I COLLAGEN ALPHA-1, LYSYL OXIDASE, AND CYCLOOXYGENASE-1 GENES IN HUMAN EMBRYO LUNG FIBROBLASTS", Journal of cellular biochemistry, 71(2), 1998, pp. 254-263

Abstract

In a recent communication, we demonstrated that prostaglandin E-2 (PGE(2)) lowers basal while it ablates interleukin-1 beta (IL-1 beta) andtransforming growth factor-beta (TGF beta) upregulated lysyl oxidase (LO) mRNA levels. Correspondingly, PGE(2) increases cvclooxygenase-1 (COX1) mRNA in diploid, human embryo lung fibroblasts (IMR90) [Roy et al., 1996]. We now report that these actions by PGE(2) are routed through cAMP via the PGE(2), EP2 receptor. Among the PGE(2) receptor types,the IMR90 predominantly express the EP2 mRNA. These cells also express EP3 and EP4 mRNA at comparatively low levels. Northern blot analysesshow that 11-deoxy PGE(1), an EP2/EP4 agonist, emulates the action ofPGE(2). In a similar manner to PGE(2) 11-deoxy PGE(1) decreases basaland TGF-beta induced type I collagen alpha 1 (COL) mRNA, basal and IL-1 beta induced LO mRNA while it increases COX1 mRNA. Sulprostone, an EP3/EP1 agonist, has no effect on the expression of these three genes. Forskolin, an adenylate cyclase activator, acts in a very similar manner to PGE(2) or 11-deoxy PGE(1). It suppresses both basal and TGF-beta induced COL mRNA levels. Both PGE(2) and 11-deoxy PGE(1) increase cAMP to a level comparable with forskolin. The role of the EP2 receptor in controlling collagen production is further underscored in the immortalized Rat-1 fibroblasts, derived from Fischer rat embryos, which do not express delectable EP2 mRNA. In these cells, PGE(2) has little Effect on COL mRNA level, whereas forskolin increases it. Furthermore, forskolin increases cAMP level in Rat-1 cells, whereas PGE(2) does not. Overall, these results illustrate that much of the PGE(2) action on the expression of COL, LO, and COX1 genes is mediated through the EP2 receptor and a subsequent increase in intracellular cAMP. J. Cell. Biochem. 71:254-263, 1998. (C) 1998 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/10/20 alle ore 21:37:20