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Titolo:
H-RAS INDUCES GLUCOSE-UPTAKE IN BROWN ADIPOCYTES IN AN INSULIN-INDEPENDENT AND PHOSPHATIDYLINOSITOL 3-KINASE-INDEPENDENT MANNER
Autore:
VALVERDE AM; NAVARRO P; BENITO M; LORENZO M;
Indirizzi:
UNIV COMPLUTENSE,FAC PHARM,DEPT BIOCHEM & MOL BIOL E-28040 MADRID SPAIN UNIV COMPLUTENSE,FAC PHARM,DEPT BIOCHEM & MOL BIOL E-28040 MADRID SPAIN
Titolo Testata:
Experimental cell research
fascicolo: 2, volume: 243, anno: 1998,
pagine: 274 - 281
SICI:
0014-4827(1998)243:2<274:HIGIBA>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWTH-FACTOR-I; 3T3-L1 ADIPOCYTES; SIGNALING PATHWAY; INDUCED DIFFERENTIATION; PRIMARY CULTURES; GENE-EXPRESSION; ADIPOSE-TISSUE; TRANSPORT; RECEPTOR; 3-KINASE;
Keywords:
INSULIN RESISTANCE; H-RAS TRANSFORMATION; GLUT4/GLUT1 MESSENGER-RNA AND PROTEIN CONTENT; GLUCOSE UPTAKE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
A.M. Valverde et al., "H-RAS INDUCES GLUCOSE-UPTAKE IN BROWN ADIPOCYTES IN AN INSULIN-INDEPENDENT AND PHOSPHATIDYLINOSITOL 3-KINASE-INDEPENDENT MANNER", Experimental cell research, 243(2), 1998, pp. 274-281

Abstract

Fetal brown adipocytes (parental cells) expressed mainly Glut4 mRNA glucose transporter, the expression of Glut1 mRNA being much lower. At physiological doses, insulin stimulation for 15 min increased 3-fold glucose uptake and doubled the amount of Glut4 protein located at the plasma membrane. Moreover, phosphatidylinositol (PI) 3-kinase activity was induced by the presence of insulin in those cells, glucose uptake being precluded by PI S-kinase inhibitors such as wortmannin or LY294002. H-ras(lys12)-transformed brown adipocytes showed a 10-fold higher expression of Glut1 mRNA and protein than parental cells, Glut4 gene expression being completely down-regulated. Glucose uptake increased by10-fold in transformed cells compared to parental cells; this uptake was unaltered in the presence of insulin and/or wortmannin. Transient transfection of parental cells with a dominant form of active Pas increased basal glucose uptake by 5-fold, no further effects being observed in the presence of insulin. However, PI 3-kinase activity (immunoprecipitated with anti-alpha p85 subunit of PI 3-kinase) remained unaltered in H-ras permanent and transient transfectants. Our results indicate that activated Pas induces brown adipocyte glucose transport in an insulin-independent manner, this induction not involving PI 3-kinase activation. (C) 1998 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/08/20 alle ore 19:34:45