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Titolo:
INSULIN IGF-I RESCUES IMMORTALIZED BROWN ADIPOCYTES FROM APOPTOSIS DOWN-REGULATING BCL-XS EXPRESSION, IN A PI 3-KINASE-DEPENDENT AND MAP KINASE-DEPENDENT MANNER/
Autore:
NAVARRO P; VALVERDE AM; BENITO M; LORENZO M;
Indirizzi:
UNIV COMPLUTENSE,FAC FARM,DEPT BIOQUIM & BIOL MOL 2 E-28040 MADRID SPAIN UNIV COMPLUTENSE,FAC FARM,DEPT BIOQUIM & BIOL MOL 2 E-28040 MADRID SPAIN
Titolo Testata:
Experimental cell research
fascicolo: 2, volume: 243, anno: 1998,
pagine: 213 - 221
SICI:
0014-4827(1998)243:2<213:IIRIBA>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWTH-FACTOR-I; ACTIVATED PROTEIN-KINASE; MYC-INDUCED APOPTOSIS; PHOSPHATIDYLINOSITOL 3-KINASE; GENE-EXPRESSION; IGF-I; INDUCED DIFFERENTIATION; SURVIVAL FACTOR; CELL-LINES; RAS;
Keywords:
IGF-I/INSULIN; PI 3-KINASE; MAPK; CELL CYCLE; APOPTOSIS; BCL-XL/S;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
42
Recensione:
Indirizzi per estratti:
Citazione:
P. Navarro et al., "INSULIN IGF-I RESCUES IMMORTALIZED BROWN ADIPOCYTES FROM APOPTOSIS DOWN-REGULATING BCL-XS EXPRESSION, IN A PI 3-KINASE-DEPENDENT AND MAP KINASE-DEPENDENT MANNER/", Experimental cell research, 243(2), 1998, pp. 213-221

Abstract

Serum deprivation of immortalized brown adipocyte cell line resulted in growth arrest in G0/G1 phases of the cell cycle and apoptosis, as detected either by DNA laddering or by increase in the percentage of hypodiploid cells. Furthermore, apoptosis is concurrent with a dramatic increase in the expression of the proapoptotic protein Bcl-xS, the expression of Bcl-xL remaining almost undetectable. Insulin/insulin-like growth factor (IGF-I) rescued serum-deprived brown adipocytes from apoptosis, decreasing the number of hypodiploid cells and increasing the number of cells undergoing cell cycle progression throughout S and G2/M phases of the cell cycle. Moreover, insulin down-regulated Bcl-xS expression without inducing the expression of Bcl-xL. Both phosphatidylinositol (PI) 3-kinase and mitogen-activated protein kinase (MAPK) pathways are necessary for insulin/IGF-I full survival effect, since the use of specific inhibitors of PI 3-kinase activity (wortmannin or LY294002, at the dose that inhibits PI 3-kinase activity induced by insulin) or MAPK kinase activity inhibitor (PD098059, at the dose that inhibits insulin-induced phosphorylation of MAPK) totally blocked the antiapoptotic effect induced by insulin/IGF-I, respectively. In conclusion, insulin survival effect on immortalized brown adipocytes is associatedwith inhibition of the Bcl-xS content without changing Bcl-xL, in a PI 3-kinase- and MAP kinase-dependent manner. (C) 1998 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/11/20 alle ore 10:51:03