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Titolo:
REGULATED LENTIVIRAL PACKAGING CELL-LINE DEVOID OF MOST VIRAL CIS-ACTING SEQUENCES
Autore:
KAUL M; YU H; RON Y; DOUGHERTY JP;
Indirizzi:
UNIV MED & DENT NEW JERSEY,ROBERT WOOD JOHNSON MED SCH,DEPT MOL GENET& MICROBIOL,675 HOES LANE PISCATAWAY NJ 08854 UNIV MED & DENT NEW JERSEY,ROBERT WOOD JOHNSON MED SCH,DEPT MOL GENET& MICROBIOL PISCATAWAY NJ 08854 RUTGERS STATE UNIV,GRAD PROGRAM MICROBIOL & MOL GENET NEW BRUNSWICK NJ 08903
Titolo Testata:
Virology (New York, N.Y. Print)
fascicolo: 1, volume: 249, anno: 1998,
pagine: 167 - 174
SICI:
0042-6822(1998)249:1<167:RLPCDO>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; GENE-TRANSFER; NONDIVIDING CELLS; MAMMALIAN-CELLS; VECTOR; EXPRESSION; INFECTION; PROTEIN; HIV-1; RNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
40
Recensione:
Indirizzi per estratti:
Citazione:
M. Kaul et al., "REGULATED LENTIVIRAL PACKAGING CELL-LINE DEVOID OF MOST VIRAL CIS-ACTING SEQUENCES", Virology (New York, N.Y. Print), 249(1), 1998, pp. 167-174

Abstract

Packaging cell lines derived from human immunodeficiency virus-1 (HIV-1) are promising tools for in vivo somatic cell gene therapy protocols due to the ability of lentiviruses to infect nondividing cells. We describe here the generation of a safe, stable HIV-1 packaging cell line capable of expressing all of the HIV-1 structural, enzymatic, and regulatory proteins but lacking majority of the cis-acting sequences. The use of an inducible expression system circumvented the cytotoxic andcytostatic effects associated with the expression of some of the HIV-1 viral proteins. Reverse transcriptase activity was detectable in thesupernatant from the stable packaging line 1 day after induction, while vector titers peaked 5 days postinduction. Vector titers of up to 3.5 x 10(4) infectious units/ml (IU/ml) were maintained through 8 months following the establishment of the cell line. Lineage-specific gene delivery can be achieved from this established cell line as viral stocks obtained specifically infect CD4(+) target cells. Moreover, this cell line provides a safe and easy to use system for screening of drugs that inhibit HIV-1 replication. (C) 1998 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 13/07/20 alle ore 11:16:23