Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
CHARACTERIZATION OF HUMAN MONOCLONAL-ANTIBODIES SPECIFIC TO THE HEPATITIS-C VIRUS GLYCOPROTEIN E2 WITH IN-VITRO BINDING NEUTRALIZATION PROPERTIES
Autore:
HABERSETZER F; FOURNILLIER A; DUBUISSON J; ROSA D; ABRIGNANI S; WYCHOWSKI C; NAKANO I; TREPO C; DESGRANGES C; INCHAUSPE G;
Indirizzi:
INSERM U271,151 COURS ALBERT THOMAS F-69003 LYON 03 FRANCE INSERM U271 F-69003 LYON 03 FRANCE INST PASTEUR,INST BIOL,CNRS UMR 319 F-59021 LILLE FRANCE IRIS I-53100 SIENA ITALY
Titolo Testata:
Virology (New York, N.Y. Print)
fascicolo: 1, volume: 249, anno: 1998,
pagine: 32 - 41
SICI:
0042-6822(1998)249:1<32:COHMST>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
B-CELL EPITOPE; HYPERVARIABLE REGION-1; STRUCTURAL PROTEINS; ENVELOPE GLYCOPROTEINS; NUCLEOCAPSID PROTEIN; RECOMBINANT VACCINIA; HYPERIMMUNE SERUM; IMMUNE-RESPONSES; HCV INFECTION; CHIMPANZEES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
51
Recensione:
Indirizzi per estratti:
Citazione:
F. Habersetzer et al., "CHARACTERIZATION OF HUMAN MONOCLONAL-ANTIBODIES SPECIFIC TO THE HEPATITIS-C VIRUS GLYCOPROTEIN E2 WITH IN-VITRO BINDING NEUTRALIZATION PROPERTIES", Virology (New York, N.Y. Print), 249(1), 1998, pp. 32-41

Abstract

Both linear and conformational determinants of hepatitis C virus (HCV) are believed to be involved in viral neutralization. After immortalization of B cells from HCV chronically infected patients with Epstein-Barr virus, we obtained two polyclonal lymphoblastoid cell lines (LCL)secreting human monoclonal antibodies (HMabs). One clone was derived from a patient infected with a genotype 4 isolate while the second wasisolated from a genotype Ib-infected patient. Immunoprecipitation studies, Western blot, and immunofluorescence analysis, peptide scanning,and ELISA studies indicated that the HMabs (1) recognized conformation-dependent determinant(s), (2) were capable of recognizing genotype la and Ib derived antigens, and (3) were able to precipitate noncovalently associated E1 E2 complexes believed to exist on the surface of virion particles. The HMab derived from the genotype LF-infected patient was in addition shown to neutralize the in vitro binding of recombinant E2 protein onto susceptible cells suggesting a potential for in vivoneutralization. These data indicate that anti-E2 antibodies directed at conserved conformational-dependent determinant(s) exist in chronic HCV infection. (C) 1998 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 08:12:42