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Titolo:
DIFFERENTIAL GENE-EXPRESSION IN APOPTOSIS - IDENTIFICATION OF RIBOSOMAL-PROTEIN 23K, A CELL-PROLIFERATION INHIBITOR
Autore:
CHEN FW; DAVIES JP; IOANNOU YA;
Indirizzi:
CUNY MT SINAI SCH MED,DEPT HUMAN GENET,BOX 1498,5TH AVE & 100TH ST NEW YORK NY 10029 CUNY MT SINAI SCH MED,DEPT HUMAN GENET NEW YORK NY 10029
Titolo Testata:
MOLECULAR GENETICS AND METABOLISM
fascicolo: 4, volume: 64, anno: 1998,
pagine: 271 - 282
SICI:
1096-7192(1998)64:4<271:DGIA-I>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
T-LYMPHOMA CELLS; MESSENGER-RNAS; HL-60 CELLS; DEATH; INDUCTION; LYMPHOCYTES; ACTIVATION; LINE; L7; PURIFICATION;
Keywords:
APOPTOSIS; L13A; DIFFERENTIAL DISPLAY; GROWTH INHIBITOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
45
Recensione:
Indirizzi per estratti:
Citazione:
F.W. Chen et al., "DIFFERENTIAL GENE-EXPRESSION IN APOPTOSIS - IDENTIFICATION OF RIBOSOMAL-PROTEIN 23K, A CELL-PROLIFERATION INHIBITOR", MOLECULAR GENETICS AND METABOLISM, 64(4), 1998, pp. 271-282

Abstract

Gene expression during the camptothecin-induced apoptotic death of human leukemic U937 cells and mouse T-cell hybridoma QW4.1 cells was studied by the mRNA differential display technique. Ten clones were confirmed to be differentially expressed, nine of which encoded novel sequences. One clone, U3.2, was induced approximately 10-fold in camptothecin-treated cells and was found to be identical to a highly basic 23-kDa human protein which contains basic leucine zipper-like motifs and has recently been identified as the human homologue of the rat ribosomalprotein L13a. Northern blot analysis revealed a major mRNA of similarto 0.9 kb and a minor mRNA of similar to 1.3 kb. Overexpression of a full-length 23K cDNA, tagged with a FLAG sequence, in COS-7 cells revealed a predominantly nucleolar localization and the absence of any 23Kprotein from the cytoplasm. Subsequent transfection studies, using antisense phosphorothioate-modified oligonucleotides, revealed that inhibition of 23K expression results in an increased cell proliferation and greater sensitivity of U937 cells to the effects of camptothecin-induced cell death. Upregulation of 23K expression using a cDNA constructresulted in a decrease in cell proliferation and growth arrest, suggesting a role for 23K protein as a proliferation checkpoint following acellular insult. (C) 1998 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 14:35:58