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Titolo:
CHOLINERGIC CELL EXPRESSION IN THE DEVELOPING RAT MEDIAL SEPTAL NUCLEUS IN-VITRO IS DIFFERENTIALLY CONTROLLED BY GABA(A) AND GABA(B) RECEPTORS
Autore:
KENIGSBERG RL; HONG YG; THEORET Y;
Indirizzi:
HOP ST JUSTINE,CTR RECH,DEPT PEDIAT,3175 COTE ST,CATHERINE RD MONTREAL PQ H3T 1C5 CANADA HOP ST JUSTINE,RES CTR,DEPT PHYSIOL MONTREAL PQ H3T 1C5 CANADA HOP ST JUSTINE,RES CTR,DEPT PHARMACOL MONTREAL PQ H3T 1C5 CANADA
Titolo Testata:
Brain research
fascicolo: 1-2, volume: 805, anno: 1998,
pagine: 123 - 130
SICI:
0006-8993(1998)805:1-2<123:CCEITD>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
GAMMA-AMINOBUTYRIC-ACID; SUBUNIT MESSENGER-RNAS; CENTRAL-NERVOUS-SYSTEM; CEREBELLAR GRANULE CELLS; CEREBRAL CORTICAL-CELLS; GROWTH-FACTOR; POSTNATAL-DEVELOPMENT; BASAL FOREBRAIN; GABA(A)-RECEPTOR SUBTYPES; HIPPOCAMPAL-NEURONS;
Keywords:
MEDIAL SEPTUM; CHOLINERGIC NEURON; GABA RECEPTOR; CHOLINE ACETYLTRANSFERASE (CHAT); CELL CULTURE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
59
Recensione:
Indirizzi per estratti:
Citazione:
R.L. Kenigsberg et al., "CHOLINERGIC CELL EXPRESSION IN THE DEVELOPING RAT MEDIAL SEPTAL NUCLEUS IN-VITRO IS DIFFERENTIALLY CONTROLLED BY GABA(A) AND GABA(B) RECEPTORS", Brain research, 805(1-2), 1998, pp. 123-130

Abstract

The early appearance and relative abundance of GABAergic neurons in basal forebrain cholinergic nuclei like the medial septum suggest that the maturation of the later developing cholinergic neurons in these nuclei may be controlled by GABA. To examine this possibility, the effects of both exogenous GABA and specific GABA receptor agonists, as wellas that of endogenous GABA on the phenotypic expression and survival of the cholinergic neurons in primary cultures from the fetal rat medial septum, were studied. Treatment of these cultures for six days withGABA significantly decreased the enzymatic activity of choline acetyltransferase (EC 2.3.1.6) (ChAT) in a dose-dependent manner. This response to exogenous GABA was blocked by bicuculline, mimicked by muscimoland slightly potentiated by saclofen. Consistent with this latter observation, the GABA(B) receptor agonist, baclofen, dose-dependently increased septal ChAT activity. However, while the effect of baclofen on cholinergic expression was lost in the absence of glia, the suppressive effects of GABA or muscimol were more marked. Acetylcholinesterase (EC 3.1.1.7) (AChE) expression in mixed neuronal-glial cultures, was, like ChAT activity, increased or decreased in intensity with the inclusion of baclofen or muscimol, respectively. Although the number of AChEpositive neurons in muscimol-treated cultures was significantly lowerthan that in controls, no changes in neither neuronal nor general cell viability were noted. Finally, as GABA(A) or GABA(B) receptor antagonists bicuculline and picrotoxin or saclofen, when applied alone to mixed cultures, increased or decreased ChAT activity, respectively, it appears that endogenous GABA, tonically released in the developing septum, may, via specific receptor types, differentially control the biochemical maturation of the cholinergic neurons. (C) 1998 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 12:15:47