Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
A HIGHLY SENSITIVE AND SPECIFIC ASSAY USING A NOVEL HUMAN GROWTH-HORMONE CDNA REPORTER GENE REGULATED BY THE HUMAN INTERLEUKIN-4 INDUCIBLE GERMLINE EPSILON-TRANSCRIPT PROMOTER
Autore:
JENH CH; COX MA; LUNDELL D; NARULA SK; ZAVODNY PJ;
Indirizzi:
SCHERING PLOUGH CORP,RES INST,DEPT IMMUNOL KENILWORTH NJ 07033
Titolo Testata:
Journal of immunological methods
fascicolo: 1-2, volume: 217, anno: 1998,
pagine: 87 - 95
SICI:
0022-1759(1998)217:1-2<87:AHSASA>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN B-CELLS; INTERFERON-ALPHA; IGE PRODUCTION; C-EPSILON; EXPRESSION; REGIONS; SWITCH; SEQUENCES; GAMMA; SITE;
Keywords:
HUMAN INTERLEUKIN-4; HUMAN GROWTH HORMONE CDNA; REPORTER GENE; GERMLINE EPSILON TRANSCRIPT PROMOTER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
C.H. Jenh et al., "A HIGHLY SENSITIVE AND SPECIFIC ASSAY USING A NOVEL HUMAN GROWTH-HORMONE CDNA REPORTER GENE REGULATED BY THE HUMAN INTERLEUKIN-4 INDUCIBLE GERMLINE EPSILON-TRANSCRIPT PROMOTER", Journal of immunological methods, 217(1-2), 1998, pp. 87-95

Abstract

We have successfully developed a highly sensitive and specific assay system for human interleukin-4 (IL-4) regulated gene expression. It isbased on a human Jijoye cell line with the germline epsilon transcript promoter joined to the human growth hormone (hGH) cDNA. The germlineepsilon transcript promoter is responsive to IL-4 and involved in immunoglobulin heavy chain class switching. We cloned hGH complementary DNA (cDNA) as the reporter gene instead of using conventional hGH genomic DNA which failed to generate any IL-4 inducible clone in human Jijoye cells. The two IL-4 inducible cell lines with the hGH cDNA reportershow high signal/noise ratio for IL-4-mediated induction (60-90 fold). The response to IL-4 is dose-dependent with ED50 of 10 pM. As expected, there is no response to other human cytokines and growth factors, as well as mouse IL-4. The mutant hIL-4 antagonist hIL-4.Y124D inhibits the induction mediated by native hIL-4. These IL-4 inducible cell Lines provide a sensitive, specific assay system to study IL-4-regulatedgene expression, and in particular the regulation of the germline epsilon promoter. (C) 1998 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 13:08:06