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Titolo:
STUDY OF THE HUMAN KERATINOCYTE ISOLATION METHODS AND IN-VITRO CULTURE TECHNIQUES IN A SINGLE LABORATORY
Autore:
FERNANDEZ EG; BEJAR JM; ALONSOVARONA A; MASDEVALL MDG; GABILONDO FJ;
Indirizzi:
HOSP CRUCES,DEPT IMMUNOL,PLAZA CRUCES S-N E-48903 BARACALDO VIZCAYA SPAIN
Titolo Testata:
European journal of plastic surgery
fascicolo: 7, volume: 21, anno: 1998,
pagine: 353 - 357
SICI:
0930-343X(1998)21:7<353:SOTHKI>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN EPIDERMAL KERATINOCYTES; GROWTH; CELLS; INVITRO;
Keywords:
KERATINOCYTE; CULTURE; GROWTH; METHODOLOGY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
29
Recensione:
Indirizzi per estratti:
Citazione:
E.G. Fernandez et al., "STUDY OF THE HUMAN KERATINOCYTE ISOLATION METHODS AND IN-VITRO CULTURE TECHNIQUES IN A SINGLE LABORATORY", European journal of plastic surgery, 21(7), 1998, pp. 353-357

Abstract

The survey was conducted to analyze different human keratinocyte isolation methods and in vitro culture techniques used in current practice. The aim was to find which would work best in our laboratory to obtain epidermal sheets suitable for grafting. The techniques for epidermalremoval and keratinocyte isolation involve dispase II or trypsin solutions at different concentrations and times. The number of cells and cellular viability were measured from different origins and size samples. The main approaches developed for in vitro culture of keratinocytesinclude the use of serum-containing media and of a feeder-layer of lethally irradiated mouse fibroblasts, serum-free medium in the absence of a feeder-layer and a commercially prepared serum-free medium. Othertechniques include the use of different substrates such as laminin, fibronectin, collagen IV or fibrin glue to obtain more complete differentiation of epithelial sheets. The growth rate and cellular attachmenthave been studied using these media and support systems. In summary, for the isolation of keratinocytes, the best results were obtained with 0.17% trypsin solution overnight. The best attachment and growth rates of cell cultures have been obtained when using serum-containing medium and a feeder-layer of lethally irradiated mouse fibroblasts.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/07/20 alle ore 08:01:42