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Titolo:
CD34(-) AND CD34(+)CD38(+) HUMAN HEMATOPOIETIC PROGENITORS FROM FETALLIVER, CORD-BLOOD, AND ADULT BONE-MARROW RESPOND DIFFERENTLY TO HEMATOPOIETIC CYTOKINES DEPENDING ON THE ONTOGENIC SOURCE(+)CD38()
Autore:
WEEKX SFA; VANBOCKSTAELE DR; PLUM J; MOULIJN A; RODRIGUS I; LARDON F; DESMEDT M; NIJS G; LENJOU M; LOQUET P; BERNEMAN ZN; SNOECK HW;
Indirizzi:
UNIV ANTWERP HOSP,LAB EXPT HEMATOL,WILRIJKSTR 10 B-2650 EDEGEM BELGIUM UNIV ANTWERP HOSP,LAB EXPT HEMATOL B-2650 EDEGEM BELGIUM UNIV ANTWERP HOSP,DEPT CARDIAC SURG B-2650 EDEGEM BELGIUM UNIV ANTWERP HOSP,DEPT OBSTET & GYNECOL B-2650 EDEGEM BELGIUM STATE UNIV GHENT HOSP,DEPT CLIN CHEM MICROBIOL & IMMUNOL B-9000 GHENTBELGIUM
Titolo Testata:
Experimental hematology
fascicolo: 11, volume: 26, anno: 1998,
pagine: 1034 - 1042
SICI:
0301-472X(1998)26:11<1034:CACHHP>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
STEM-CELLS; YOLK-SAC; MOUSE; TRANSPLANTATION; EXPRESSION; POPULATION; GROWTH; CD38; IDENTIFICATION; SUBPOPULATIONS;
Keywords:
HEMATOPOIESIS; FETAL LIVER; CORD BLOOD; ADULT BONE MARROW; INTERFERON-GAMMA; MACROPHAGE INFLAMMATORY PROTEIN-1-ALPHA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
41
Recensione:
Indirizzi per estratti:
Citazione:
S.F.A. Weekx et al., "CD34(-) AND CD34(+)CD38(+) HUMAN HEMATOPOIETIC PROGENITORS FROM FETALLIVER, CORD-BLOOD, AND ADULT BONE-MARROW RESPOND DIFFERENTLY TO HEMATOPOIETIC CYTOKINES DEPENDING ON THE ONTOGENIC SOURCE(+)CD38()", Experimental hematology, 26(11), 1998, pp. 1034-1042

Abstract

CD34(++)CD38(-) and CD34(+)CD38(+) hematopoietic progenitor cells (HPCs) from human fetal liver (FL), cord blood (CB), and adult bone marrow (ABM) were isolated and investigated for their growth characteristics, cytokine requirements and response to two modulators of early hematopoiesis, interferon (IFN)-gamma and macrophage inflammatory protein (MIP)-1 alpha. We observed first that a significantly lower percentage of CD34(++) cells were CD38(-) in ABM than in FL and CB. Second, the functional differences between CD34(++)CD38(-) and CD34(+)CD38(+) cellswere less pronounced in FL and CB than in their ABM counterparts. Third, an inverse correlation was found between growth factor response and the ontogenic age of KPCs, and a direct correlation was noted between cytokine requirements and the ontogenic age of HPCs. Fourth, spontaneous colony formation in a classic semisolid culture system was reproducibly obtained only in the ontogenically earliest cells, that is, in FL but not in CB and ABM, in which no such spontaneous colony formation was observed. Fifth, the modulatory effects of IFN-gamma and MIP-1 alpha were qualitatively different depending on the ontogenic age of the progenitor source: whereas IFN-gamma was only a selective inhibitor of primitive CD34(++)CD38(-) ABM progenitor cells, it inhibited both CD34(++)CD38(-) and CD34(+)CD38(+) FL and CB cells to the same extent. In contrast to the effects of MIP-1 alpha on ABM, we could not find any consistently stimulatory or inhibitory effects on FL and CB progenitors. In conclusion, important functional and biologic differences exist between FL, CB, and ABM progenitor cells, and these differences could have major implications for the use of these cell populations in preparative protocols of ex vivo expansion, transplantation strategies, or gene transfer experiments.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 11:40:34