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Titolo:
DEVELOPMENTAL-CHANGES IN THE EXPRESSION OF THE GROWTH-HORMONE RECEPTOR MESSENGER-RIBONUCLEIC-ACID AND PROTEIN IN THE BOVINE OVARY
Autore:
KOLLE S; SINOWATZ F; BOIE G; LINCOLN D;
Indirizzi:
UNIV MUNICH,DEPT VET ANAT 2,VET STR 13 D-80539 MUNICH GERMANY KUWAIT UNIV,FAC ALLIED HLTH SCI SULIBIKHAT 90805 KUWAIT
Titolo Testata:
Biology of reproduction
fascicolo: 4, volume: 59, anno: 1998,
pagine: 836 - 842
SICI:
0006-3363(1998)59:4<836:DITEOT>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
FOLLICLE-STIMULATING-HORMONE; HUMAN GRANULOSA-CELLS; IN-VITRO MATURATION; BINDING-PROTEIN; INDUCED DIFFERENTIATION; MOLECULAR-CLONING; LOCALIZATION; SOMATOTROPIN; RNA; STEROIDOGENESIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
S. Kolle et al., "DEVELOPMENTAL-CHANGES IN THE EXPRESSION OF THE GROWTH-HORMONE RECEPTOR MESSENGER-RIBONUCLEIC-ACID AND PROTEIN IN THE BOVINE OVARY", Biology of reproduction, 59(4), 1998, pp. 836-842

Abstract

By reverse transcription-polymerase chain reaction, the transcript ofthe growth hormone receptor (GHR) was demonstrated in oocytes, follicular cells, and corpus luteum of the bovine ovary. Immunoblotting using the monoclonal antibody mAb 263 resulted in a distinct protein band at 120 kDa, confirming that translation of the mRNA takes place in thesame cells. Nonradioactive in situ hybridization revealed that distribution of the mRNA encoding GHR was correlated with the developmental stage of the follicle. Whereas in primordial and primary follicles theoocyte showed distinct amounts of the transcript encoding GHR, in tertiary follicles the mRNA was predominantly localized in the cells of the cumulus oophorus. GHR mRNA was also expressed in the large granulosa lutein cells, in the germinal epithelium, and in the endothelial cells of ovarian vessels. Colocalization of the GHR protein showed a distribution pattern identical to that of the mRNA. In carves, oocyte and follicle cells changed GHR expression in the same way as in the adult ovary. During embryonic development of the ovary, distinct amounts of the mRNA encoding GHR were found in primordial follicles shortly before birth. Our results imply that the GHR is involved in ovarian ontogenesis, especially in early folliculogenesis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 13:31:41