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Titolo:
DEXAMETHASONE MODULATES THE EXPRESSION OF ENDOTHELIN-1 AND ENDOTHELIN-A RECEPTORS IN A7R5 VASCULAR SMOOTH-MUSCLE CELLS
Autore:
KOSHINO Y; HAYASHI T; MATSUKAWA S; ASAZUMA K; EGUCHI K; KATO H; NAKAI T; MIYAMORI I;
Indirizzi:
FUKUI MED SCH,DEPT INTERNAL MED 3,23 SHIMOAIZUKI,MATSUOKA CHO FUKUI 91011 JAPAN FUKUI MED SCH,DEPT INTERNAL MED 3 FUKUI 91011 JAPAN FUKUI MED SCH,CENT RES LABS FUKUI 91011 JAPAN
Titolo Testata:
Journal of cardiovascular pharmacology
fascicolo: 4, volume: 32, anno: 1998,
pagine: 665 - 672
SICI:
0160-2446(1998)32:4<665:DMTEOE>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RNA EXPRESSION; AUTOCRINE PRODUCTION; DOWN-REGULATION; CA2+ INFLUX; GLUCOCORTICOIDS; BINDING; PROLIFERATION; INHIBITION; ANTAGONIST; GENERATION;
Keywords:
DEXAMETHASONE; ENDOTHELIN; [I-125] ENDOTHELIN-1 BINDING; FR139317; INTRACELLULAR FREE CA2+; VASCULAR SMOOTH-MUSCLE CELL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
29
Recensione:
Indirizzi per estratti:
Citazione:
Y. Koshino et al., "DEXAMETHASONE MODULATES THE EXPRESSION OF ENDOTHELIN-1 AND ENDOTHELIN-A RECEPTORS IN A7R5 VASCULAR SMOOTH-MUSCLE CELLS", Journal of cardiovascular pharmacology, 32(4), 1998, pp. 665-672

Abstract

Endothelin-l (ET-1) is synthesized and released by vascular smooth-muscle cells (VSMCs). Glucocorticoids induce the release of ET-1 from VSMCs into the medium. We investigated whether glucocorticoids modulate ET-1 action by an autocrine production of ET-1 in A7r5 VSMCs. Dexamethasone (100 nM) stimulated the release of ET-1 into the medium. Treatment with 100 nM dexamethasone for 24 h reduced the peak increase of intracellular free Ca2+ induced by ET-1 (100 nM) by 50%, an effect that was dose-dependently inhibited by the specific ETA-receptor antagonist FR139317. Scatchard plots of [(125)]-ET-1 binding revealed that dexamethasone reduced the number of maximal ET-1 binding sites without altering their binding affinity. FR139317 reversed the decrease in ET-1 binding capacity induced by dexamethasone. Northern blot analysis revealed that dexamethasone increased the level of prepro-ET-l messenger RNA (mRNA) and decreased the level of ETA-receptor mRNA. FR139317 prevented the decrease in the level of ETA-receptor mRNA induced by dexamethasone. Results indicate that dexamethasone downregulates ETA receptors in A7r5 VSMCs at the mRNA level, in part by the autocrine production ofET-1.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 14:15:02