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Titolo:
INCREASED LEVELS OF ENDOTHELIN ETB RECEPTOR MESSENGER-RNA IN HUMAN OMENTAL ARTERIES AFTER ORGAN-CULTURE - QUANTIFICATION BY COMPETITIVE REVERSE TRANSCRIPTION-POLYMERASE CHAIN-REACTION
Autore:
MOLLER S; ADNER M; EDVINSSON L;
Indirizzi:
UNIV LUND HOSP,DEPT CELL BIOL 1,EB BLOCKET S-22185 LUND SWEDEN UNIV LUND HOSP,DEPT INTERNAL MED,DIV EXPT VASC RES S-22185 LUND SWEDEN
Titolo Testata:
Clinical and experimental pharmacology and physiology
fascicolo: 10, volume: 25, anno: 1998,
pagine: 788 - 794
SICI:
0305-1870(1998)25:10<788:ILOEER>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN BLOOD-VESSELS; CONTRACTILE ENDOTHELIN; ET(B) RECEPTORS; MESSENGER-RNA; B RECEPTORS; HUMAN RESISTANCE; ET(A); CLONING; VASOCONSTRICTION; QUANTITATION;
Keywords:
COMPETITIVE REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; ENDOTHELIN; ENDOTHELIN RECEPTORS; HUMAN OMENTAL ARTERIES; IRL 1620; MESSENGER-RNA; ORGAN CULTURE; QUANTIFICATION; RECEPTOR UP-REGULATION; SMOOTH MUSCLE CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
S. Moller et al., "INCREASED LEVELS OF ENDOTHELIN ETB RECEPTOR MESSENGER-RNA IN HUMAN OMENTAL ARTERIES AFTER ORGAN-CULTURE - QUANTIFICATION BY COMPETITIVE REVERSE TRANSCRIPTION-POLYMERASE CHAIN-REACTION", Clinical and experimental pharmacology and physiology, 25(10), 1998, pp. 788-794

Abstract

1. Using competitive reverse transcription-polymerase chain reaction (RT-PCR) and in vitro pharmacology, smooth muscle endothelin ETB receptor expression was studied in segments of human omental artery, fresh and after organ culture for 1 and 5 days. 2. The competitive RT-PCR assay used in the present study uses an internal RNA standard bearing a 69 b.p. deletion in order to control all steps of the reaction, including the RT step. Control experiments showed linearity over five subsequent 1:10 dilutions and a wide range of cycle numbers. The assay was able to quantify subattomolar concentrations in samples under 1 mu g total RNA, making it possible to measure mRNA expression even in small tissue biopsies. 3. In fresh arteries, ETB mRNA levels were 0.19+/-0.05amol/mu g total RNA (range 0.03-0.42 amol/mu g; n = 8). After organ culture, an increase in ETB mRNA levels by 317+/-28 and 288+/-12% was found at days 1 and 5, compared with fresh arteries, respectively. 4. In vitro pharmacology showed that endothelin (ET)-1 induced a strong and potent contraction in fresh arteries, whereas the selective ETB receptor agonist IRI, 1620 failed to induce a significant contraction. TheET-1-induced contraction was not altered in potency or E-max after organ culture for 1 and 5 days. In contrast, IRL 1620 induced a clear contraction after 1 day, which increased further in both E-max and potency after 5 days organ culture. 5. Our results indicate that a massive new transcription of ETB receptor mRNA is induced by organ culture, resulting in functional contractile ETB receptors on the smooth muscle layer.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 19:15:03