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Titolo:
DIFFERENTIAL RESPONSE OF CD34(-BLOOD AND BONE-MARROW TO MIP-1-ALPHA AND THE EXPRESSION OF MIP-1-ALPHA RECEPTORS ON THESE IMMATURE CELLS() CELLS ISOLATED FROM CORD)
Autore:
DEWYNTER EA; DURIG J; CROSS MA; HEYWORTH CM; TESTA NG;
Indirizzi:
PATERSON INST CANC RES,CRC,SECT HAEMOPOIET CELL & GENE THERAPEUT,WILMSLOW RD MANCHESTER M20 4BX LANCS ENGLAND
Titolo Testata:
Stem cells
fascicolo: 5, volume: 16, anno: 1998,
pagine: 349 - 356
SICI:
1066-5099(1998)16:5<349:DROCAB>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
MACROPHAGE INFLAMMATORY PROTEIN-1-ALPHA; MYELOID PROGENITOR CELLS; C CHEMOKINE RECEPTOR; MOLECULAR-CLONING; FUNCTIONAL EXPRESSION; HEMATOPOIETIC-CELLS; UMBILICAL-CORD; PROTEIN 1-ALPHA; PROLIFERATION; INHIBITION;
Keywords:
MIP-1-ALPHA RECEPTORS; CHEMOKINES; HEMATOPOIETIC CELLS; CELL CYCLE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
32
Recensione:
Indirizzi per estratti:
Citazione:
E.A. Dewynter et al., "DIFFERENTIAL RESPONSE OF CD34(-BLOOD AND BONE-MARROW TO MIP-1-ALPHA AND THE EXPRESSION OF MIP-1-ALPHA RECEPTORS ON THESE IMMATURE CELLS() CELLS ISOLATED FROM CORD)", Stem cells, 16(5), 1998, pp. 349-356

Abstract

Macrophage inflammatory protein-1 alpha (MIP-1 alpha) has been shown to have a role in the control of myeloid stem and progenitor cell proliferation. Recent evidence suggests that MIP-1 alpha also has a stimulatory effect on proliferation of mature progenitors as well as an inhibitory effect on immature progenitors in vitro, We have compared the effect of MIP-1 alpha on myeloid and erythroid colony formation of CD34(+) cells isolated from bone marrow and cord blood, In the presence ofMIP-1 alpha, bone marrow granulocyte-macrophage colony forming cells (GM-CFC) were inhibited over a dose range of 15 ng/ml to 500 ng/ml, and GM-CFC from cord blood CD34(+) cells were stimulated over the same dose range, MIP-1 alpha suppressed BFU-E colonies in both bone marrow and cord blood. Using thymidine suicide assays, the influence of MIP-1 alpha on the cycling status of the cells was assessed. A good correlation between the effect of MIP-1 alpha on colony formation and cell cycle progression was observed. These results suggest that there is a differential response to MIP-1 alpha when bone marrow and cord blood CD34(+) cells are compared, Using flow cytometry and a biotinylated human MIP-1 alpha/avidin fluorescein conjugate, the expression of MIP-1 alpha receptors on CD34(+) cells was assessed. The data indicated that there was little quantitative difference in overall expression of receptors (82.9% versus 93%) from bone marrow or cord blood, respectively. However, when Northern blot analysis was used, mRNA for two different MIP-la receptors CCR1 and CCR5 could be detected in bone marrow, but only CCR1 mRNA was seen in cord blood CD34(+) samples. Therefore, the expression of different receptor subtypes on CD34(+) cells may be responsible for the difference in MIP-1 alpha responsiveness observed.

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Documento generato il 24/09/20 alle ore 03:23:31