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Titolo:
MITOTIC INACTIVATION OF A HUMAN SWI SNF CHROMATIN REMODELING COMPLEX/
Autore:
SIF S; STUKENBERG PT; KIRSCHNER MW; KINGSTON RE;
Indirizzi:
MASSACHUSETTS GEN HOSP,DEPT MOL BIOL BOSTON MA 02114 MASSACHUSETTS GEN HOSP,DEPT MOL BIOL BOSTON MA 02114 HARVARD UNIV,SCH MED,DEPT GENET BOSTON MA 02115 HARVARD UNIV,SCH MED,DEPT CELL BIOL BOSTON MA 02115
Titolo Testata:
Genes & development
fascicolo: 18, volume: 12, anno: 1998,
pagine: 2842 - 2851
SICI:
0890-9369(1998)12:18<2842:MIOAHS>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
TATA-BINDING-PROTEIN; CELL-CYCLE; TRANSCRIPTIONAL ACTIVATORS; MULTISUBUNIT COMPLEX; NUCLEOSOMAL DNA; GENE-PRODUCTS; HUMAN HOMOLOG; YEAST; COMPONENTS; STIMULATION;
Keywords:
SWI/SNF COMPLEX; NUCLEOSOMES; PHOSPHORYLATION; MITOSIS; CHROMATIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
48
Recensione:
Indirizzi per estratti:
Citazione:
S. Sif et al., "MITOTIC INACTIVATION OF A HUMAN SWI SNF CHROMATIN REMODELING COMPLEX/", Genes & development, 12(18), 1998, pp. 2842-2851

Abstract

During mitosis, chromatin is condensed into mitotic chromosomes and transcription is inhibited, processes that might be opposed by the chromatin remodeling activity of the SWI/SNF complexes. Brg1 and hBrm, which are components of human SWI/SNF (hSWIISNF) complexes, were recentlyshown to be phosphorylated during mitosis. This suggested that phosphorylation might be used as a switch to modulate SWI/SNF activity. Using an epitope-tag strategy, we have purified hSWI/SNF complexes at different stages of the cell cycle, and found that hSWI/SNF was inactive in cells blocked in G(2)-M. Mitotic hSWI/SNF contained Brg1 but trot hBrm, and was phosphorylated on at least two subunits, hSWI3 and Brg1. In vitro, active hSWI/SNF from asynchronous cells can be phosphorylatedand inactivated by ERK1, and reactivated by dephosphorylation. hSWI/SNF isolated as cells traversed mitosis regained activity when its subunits were dephosphorylated either in vitro or in vivo. We propose thatthis transitional inactivation and reactivation of hSWI/SNF is required for formation of a repressed chromatin structure during mitosis andreformation of an active chromatin structure as cells leave mitosis.

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Documento generato il 20/01/21 alle ore 03:13:52