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Titolo:
COMPARISON OF ELISA AND 2-META ASSAYS USED IN SEROLOGICAL DIAGNOSIS OF INFECTION WITH ACTINOBACILLUS-PLEUROPNEUMONIAE SEROTYPE-2 AND SEROTYPE-4-7 IN BREEDING PIGS IN CROATIA
Autore:
HABRUN B; BILIC V; HUMSKI A;
Indirizzi:
CROATIAN VET INST,DEPT BACTERIOL,SAVSKA C 143,POB 883 ZAGREB 10000 CROATIA
Titolo Testata:
Preventive veterinary medicine
fascicolo: 3, volume: 36, anno: 1998,
pagine: 179 - 186
SICI:
0167-5877(1998)36:3<179:COEA2A>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUBE AGGLUTINATION-TEST; 2-MERCAPTOETHANOL;
Keywords:
SWINE PLEUROPNEUMONIA; SEROLOGICAL DIAGNOSIS; ELISA; 2-MERCAPTOETHANOL MICROAGGLUTINATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
16
Recensione:
Indirizzi per estratti:
Citazione:
B. Habrun et al., "COMPARISON OF ELISA AND 2-META ASSAYS USED IN SEROLOGICAL DIAGNOSIS OF INFECTION WITH ACTINOBACILLUS-PLEUROPNEUMONIAE SEROTYPE-2 AND SEROTYPE-4-7 IN BREEDING PIGS IN CROATIA", Preventive veterinary medicine, 36(3), 1998, pp. 179-186

Abstract

The aim of the study was to compare the results of ELISA and 2-META assays in serological diagnosis of swine pleuropneumonia in breeding pigs of different ages. A total of 874 sera were examined for the presence of antibodies against Actinobacillus (A.) pleuropneumoniae serotype2. The sera had been taken from 437 gilts (aged approximate to 6 months), 334 sows (>2 years of age) and 104 boars (1-2 years). Sixty-nine sera of the gilts and sows, and 46 sera of the boars were also examined for the presence of antibodies against A. pleuropneumoniae serotype 4-7. Sera from the youngest age group (gilts) showed the highest agreement between the results obtained by the two assays, with a kappa value of 0.633 and 0.437 for detection of antibodies against A. pleuropneumoniae serotype 2 and 4-7, respectively. The lowest agreement of the two assays was recorded in the oldest age group (sows), with a kappa value of 0.128 and 0.199 for detection of antibodies against A. pleuropneumoniae serotype 2 and 4-7, respectively. Boars were older than giltsand younger than sows. In this group of animals, agreement between the results of the two assays was inferior to that found in gilts and superior to that recorded in sows. (C) 1998 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 23:47:01