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Titolo:
THE PRO-PROTEIN CONVERTASE PC1 IS INDUCED IN THE TRANSECTED SCIATIC-NERVE AND IS PRESENT IN CULTURED SCHWANN-CELLS - COMPARISON WITH PC5, FURIN AND PC7, IMPLICATION IN PRO-BDNF PROCESSING
Autore:
MARCINKIEWICZ M; SAVARIA D; MARCINKIEWICZ J;
Indirizzi:
UNIV MONTREAL,CLIN RES INST MONTREAL,LAB MOL NEUROENDOCRINOL,110 PINEAVE W MONTREAL PQ H2W 1R7 CANADA
Titolo Testata:
Molecular brain research
fascicolo: 2, volume: 59, anno: 1998,
pagine: 229 - 246
SICI:
0169-328X(1998)59:2<229:TPCPII>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWTH-FACTOR PRECURSOR; NEUROTROPHIC FACTOR; PROPROTEIN CONVERTASE; MESSENGER-RNA; PROHORMONE CONVERTASES; PERIPHERAL-NERVE; BRAIN; NGF; LOCALIZATION; EXPRESSION;
Keywords:
NGF; BDNF; NORTHERN BLOT; IN SITU HYBRIDIZATION; WESTERN BLOT; IMMUNOCYTOCHEMISTRY; CELL CULTURE; VACCINIA VIRUS INFECTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
41
Recensione:
Indirizzi per estratti:
Citazione:
M. Marcinkiewicz et al., "THE PRO-PROTEIN CONVERTASE PC1 IS INDUCED IN THE TRANSECTED SCIATIC-NERVE AND IS PRESENT IN CULTURED SCHWANN-CELLS - COMPARISON WITH PC5, FURIN AND PC7, IMPLICATION IN PRO-BDNF PROCESSING", Molecular brain research, 59(2), 1998, pp. 229-246

Abstract

Injury of peripheral nerves induces expression of several pro-proteinconvertases (PCs) involved in processing of precursor proteins into their diverse active end-products. In this study, the focus was on convertase PC1 which, although undetectable in control nerves, is stronglyinduced in injured nerves. High concentrations of PC1 mRNA of 9.0, 5.5, 3.0, 2.5 and 1.6 kb were observed on day 4 post-lesion in proximal and distal segments. By in situ hybridization PC1 mRNA was detected inmost of endoneurial cells, which were further identified by immunocytochemistry as myelin 2',3'-cyclic nucleotide 3'-phosphodiesterase containing Schwann cells. PC1 mRNA and protein were also present in cultured Schwann cells also containing convertases PC5, furin and PC7 as well as nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF). Mostly unprocessed pro-NGF of 35 kDa and pro-BDNF of 35 kDa were found on Western blotting of Schwann cells. Expression of exogenous neurotrophins by infection with vaccinia virus vector showed that mouse pro-NGF and rat pro-BDNF are cleaved intracellularly on smaller forms of 13.5 kDa NGF and 14 kDa BDNF. Infection experiments demonstrated that Schwann cells contain active processing enzymes. In conclusion, this work provides in vivo evidence of the presence of several PCs in the injured rat sciatic nerve and ex vivo in cultured Schwann cells. (C) 1998 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 07:33:42