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Titolo:
THE XENOPUS CHK1 PROTEIN-KINASE MEDIATES A CAFFEINE-SENSITIVE PATHWAYOF CHECKPOINT CONTROL IN CELL-FREE-EXTRACTS
Autore:
KUMAGAI A; GUO ZJ; EMAMI KH; WANG SX; DUNPHY WG;
Indirizzi:
CALTECH,HOWARD HUGHES MED INST,DIV BIOL,216-76 PASADENA CA 91125 CALTECH,HOWARD HUGHES MED INST,DIV BIOL PASADENA CA 91125
Titolo Testata:
The Journal of cell biology
fascicolo: 6, volume: 142, anno: 1998,
pagine: 1559 - 1569
SICI:
0021-9525(1998)142:6<1559:TXCPMA>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
DNA-DAMAGE CHECKPOINT; FISSION YEAST; CDC25 PROTEIN; REPLICATION CHECKPOINT; MAMMALIAN-CELLS; CYCLE ARREST; EGG EXTRACTS; PHOSPHORYLATION; MITOSIS; PHOSPHATASE;
Keywords:
CDC2; CHK1; 14-3-3 PROTEINS; CDC25; CAFFEINE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
54
Recensione:
Indirizzi per estratti:
Citazione:
A. Kumagai et al., "THE XENOPUS CHK1 PROTEIN-KINASE MEDIATES A CAFFEINE-SENSITIVE PATHWAYOF CHECKPOINT CONTROL IN CELL-FREE-EXTRACTS", The Journal of cell biology, 142(6), 1998, pp. 1559-1569

Abstract

We have analyzed the role of the protein kinase Chk1 in checkpoint control by using cell-free extracts from Xenopus eggs. Recombinant Xenopus Chk1 (Xchk1) phosphorylates the mitotic inducer Cdc25 in vitro on multiple sites including Ser-287. The Xchk1-catalyzed phosphorylation of Cdc25 on Ser-287 is sufficient to confer the binding of 14-3-3 proteins. Egg extracts from which Xchk1 has been removed by immunodepletionare strongly but not totally compromised in their ability to undergo a cell cycle delay in response to the presence of unreplicated DNA. Cdc25 in Xchk1-depleted extracts remains bound to 14-3-3 due to the action of a distinct Ser-287-specific kinase in addition to Xchk1.Xchk1 ishighly phosphorylated in the presence of unreplicated or damaged DNA,and this phosphorylation is abolished by caffeine, an agent which attenuates checkpoint control. The checkpoint response to unreplicated DNA in this system involves both caffeine-sensitive and caffeine-insensitive steps. Our results indicate that caffeine disrupts the checkpointpathway containing Xchk1.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 05:31:12