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Titolo:
TRANSCRIPTIONAL REGULATION OF BMP-4 IN THE XENOPUS EMBRYO - ANALYSIS OF GENOMIC BMP-4 AND ITS PROMOTER
Autore:
KIM J; AULT KT; CHEN HD; XU RH; ROH DH; LIN MC; PARK MJ; KUNG HF;
Indirizzi:
NCI,LAB BIOCHEM PHYSIOL,FREDERICK CANC RES & DEV CTR FREDERICK MD 21702 NCI,LAB BIOCHEM PHYSIOL,FREDERICK CANC RES & DEV CTR FREDERICK MD 21702 SCI APPLICAT INT CORP,INTRAMURAL RES SUPPORT PROGRAM FREDERICK MD 21702 NIDDKD,LAB BIOCHEM & GENET BETHESDA MD 20892 KYUNG POOK NATL UNIV,DEPT ANAT TAEGU SOUTH KOREA
Titolo Testata:
Biochemical and biophysical research communications (Print)
fascicolo: 2, volume: 250, anno: 1998,
pagine: 516 - 530
SICI:
0006-291X(1998)250:2<516:TROBIT>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
BONE MORPHOGENETIC PROTEIN-4; TGF-BETA FAMILY; HOMEOBOX GENE; DROSOPHILA EMBRYO; VENTRAL MESODERM; NEURAL INDUCTION; MARGINAL ZONE; RECEPTOR; SIGNALS; ORGANIZER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
46
Recensione:
Indirizzi per estratti:
Citazione:
J. Kim et al., "TRANSCRIPTIONAL REGULATION OF BMP-4 IN THE XENOPUS EMBRYO - ANALYSIS OF GENOMIC BMP-4 AND ITS PROMOTER", Biochemical and biophysical research communications (Print), 250(2), 1998, pp. 516-530

Abstract

Recent experiments in the Xenopus embryo suggest that proper regulation of BMP-4 signaling is critical to the dorsal ventral specification of both mesoderm and ectoderm. Regulation of BMP-4 signaling is known to occur extracellularly by direct binding with chordin, noggin, and follistatin, and intracellularly through the antagonistic signal interaction with dorsalizing TGF-beta family member activin. However, the tight repressional regulation of BMP transcription may also be required to sustain the dorsal and neural status of the induced cells. Here we demonstrate that the dominant negative mutant of the BMP receptor (DN-BR) or the BMP-4 antagonizers, chordin and noggin, negatively regulateBMP-4 transcription in animal cap explants. We suggest that repression of BMP-4 transcription is important in the maintenance of dorsal fate and that continuous input of BMP-4 signaling is required to sustain the expression of BMP-4 transcription in the maintenance of epidermal/ventral fate. Consistent with this postulation, we found that the promoter region of the isolated BMP-4 genomic DNA includes several consensus binding sites for transcriptional regulators functioning under BMP-4 signaling such as GATA binding and ventralizing homeobox genes. In afunctional assay we found that the GATA binding and ventral homeobox proteins can positively modulate BMP-4 promoter activity. We also observed that DN-BR decreases BMP-4 promoter activity. This was likely dueto a repression of the above-mentioned transcription factors. The significance of these observations to embryonic patterning is discussed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 18:40:35