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Titolo:
PURIFICATION AND CHARACTERIZATION OF CYSTATHIONINE GAMMA-LYASE FROM LACTOBACILLUS-FERMENTUM DT41
Autore:
SMACCHI E; GOBBETTI M;
Indirizzi:
UNIV PERUGIA,FAC AGR,INST DAIRY MICROBIOL,S COSTANZO I-06126 PERUGIA ITALY UNIV PERUGIA,FAC AGR,INST DAIRY MICROBIOL I-06126 PERUGIA ITALY
Titolo Testata:
FEMS microbiology letters
fascicolo: 2, volume: 166, anno: 1998,
pagine: 197 - 202
SICI:
0378-1097(1998)166:2<197:PACOCG>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
LACTOCOCCUS-LACTIS SUBSP; FLAVOR COMPOUND FORMATION; AMINO-ACIDS; BETA-CYSTATHIONASE; ESCHERICHIA-COLI; CHEESE FLAVOR; CREMORIS B78; BACTERIA;
Keywords:
AMINO ACID CATABOLISM; LACTOBACILLUS FERMENTUM; CYSTATHIONINE GAMMA-LYASE; CHEESE RIPENING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
19
Recensione:
Indirizzi per estratti:
Citazione:
E. Smacchi e M. Gobbetti, "PURIFICATION AND CHARACTERIZATION OF CYSTATHIONINE GAMMA-LYASE FROM LACTOBACILLUS-FERMENTUM DT41", FEMS microbiology letters, 166(2), 1998, pp. 197-202

Abstract

A homo-tetrameric ca. 140-kDa cystathionine gamma-lyase was purified to homogeneity from Lactobacillus fermentum DT41 by four chromatographic steps. This was the first enzyme responsible for amino acid catabolism purified from lactobacilli. The activity is pyridoxal-5'-phosphatedependent and the enzyme catalyzes the a,alpha,gamma-elimination reaction of L-cystathionine producing L-cysteine, ammonia and alpha-ketobutyrate. The cystathionine gamma-lyase produced a free thiol group, a keto acid component and ammonia from several amino acids, including L-cysteine and methionine, and amino acid derivatives. L-Cystine was the best substrate. The enzyme was stable in the conditions of cheese ripening and may contribute to the biosynthesis of sulfur-containing compounds. (C) 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 08:14:41