Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
EFFECTS OF ALDRIN ON MEMBRANE FLUIDITY AND ITS IMPLICATIONS FOR THE MECHANISMS OF TOXICITY
Autore:
DEMETRIO RS; ANTUNESMADEIRA MD; MADEIRA VMC;
Indirizzi:
CTR NEUROCIENCIAS,DEPT ZOOL P-3000 COIMBRA PORTUGAL CTR NEUROCIENCIAS,DEPT ZOOL P-3000 COIMBRA PORTUGAL
Titolo Testata:
Medical science research
fascicolo: 8, volume: 26, anno: 1998,
pagine: 557 - 561
SICI:
0269-8951(1998)26:8<557:EOAOMF>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
NATIVE MEMBRANES; FLUORESCENT-PROBES; LIPID BILAYER; DIPHENYLHEXATRIENE; PARTITION; INSECTICIDES; POLARIZATION; PROTEINS; BINDING; LINDANE;
Keywords:
ALDRIN; FLUORESCENT PROBES; PHASE TRANSITION; CHOLESTEROL; MEMBRANE FLUIDITY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
32
Recensione:
Indirizzi per estratti:
Citazione:
R.S. Demetrio et al., "EFFECTS OF ALDRIN ON MEMBRANE FLUIDITY AND ITS IMPLICATIONS FOR THE MECHANISMS OF TOXICITY", Medical science research, 26(8), 1998, pp. 557-561

Abstract

There is evidence that aldrin toxicity is initiated at the level of the cell membrane. Therefore its interaction with membrane components is of significant biochemical interest in efforts to clarify its precise mechanism of action. Thus we have studied the effects of aldrin on the physical state of model membranes by fluorescence polarization of DPH (1,6-diphenyl-1,3,5-hexatriene) and of its propionic acid derivative DPH-PA, which indicate fluidity changes in the bilayer core and in the outer regions of the bilayer respectively. Aldrin (100 mu M) increased, although to a limited extent, the fluorescence polarization of DPH and DPH-PA in fluid models of egg phosphatidylcholine, implicating areduced motion of phospholipid acyl chains, i.e. a decrease of fluidity. In agreement with data for fluid models of egg lecithin, aldrin also decreased the fluidity in the fluid phase of dimyristoylphosphatidycholine (DMPC). On the other hand, the fluorescence polarization of DPH and DPH-PA showed that the insecticide increased membrane fluidity in the gel phase of DMPC bilayers. Additionally, the phase transition profiles of DMPC vesicles treated with aldrin were broadened as compared to controls. This indicates that the cooperativity of the transitionwas affected by the insecticide. Furthermore, aldrin exerted fluidizing effects i.e. it increased the motion of phospholipid acyl chains, in DMPC bilayers enriched with cholesterol. Therefore, aldrin has specific effects on the structural properties of different membrane phases. These changes in fluidity, if also exerted in lipid domains of nativemembrane, may perturb membrane functions in association with aldrin toxicity. Med Sci Res 26:557-561 (C) 1998 Lippincott Williams & Wilkins.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 13/07/20 alle ore 17:57:44