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Titolo:
MEASURABLE HUMAN-MILK FOLATE IS INCREASED BY TREATMENT WITH ALPHA-AMYLASE AND PROTEASE IN ADDITION TO FOLATE CONJUGASE
Autore:
LIM HS; MACKEY AD; TAMURA T; WONG SC; PICCIANO MF;
Indirizzi:
PENN STATE UNIV,DEPT NUTR UNIVERSITY PK PA 16802 PENN STATE UNIV,DEPT NUTR UNIVERSITY PK PA 16802 CHONNAM NATL UNIV,DEPT FOOD & NUTR KWANGJU 500757 SOUTH KOREA UNIV ALABAMA,DEPT NUTR SCI BIRMINGHAM AL 35294
Titolo Testata:
Food chemistry
fascicolo: 3, volume: 63, anno: 1998,
pagine: 401 - 407
SICI:
0308-8146(1998)63:3<401:MHFIIB>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
FOLIC-ACID; VITAMIN-C; MICROBIOLOGICAL ASSAY; HYDROLASE CONJUGASE; FOLACIN LEVELS; BREAST-MILK; FOOD FOLATE; EXTRACTION; INFANTS; WOMEN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
H.S. Lim et al., "MEASURABLE HUMAN-MILK FOLATE IS INCREASED BY TREATMENT WITH ALPHA-AMYLASE AND PROTEASE IN ADDITION TO FOLATE CONJUGASE", Food chemistry, 63(3), 1998, pp. 401-407

Abstract

The application of trienzyme treatment prior to microbiological assayusing Lactobacillus casei for the measurement of folate in human milkwas evaluated. Trienzyme treatment consisted of alpha-amylase, protease, and folate conjugase. Human milk samples (n = 84) were heat treated for 5 min at 100 degrees C in 0.1 M potassium phosphate buffer containing 0.05 mol liter(-1) ascorbic acid, pH 4.1, then incubated with the enzymes by stepwise addition. Following trienzyme treatment, folate was measured microbiologically using a 96-well microplate method. The results show that treatment at 37 degrees C with alpha-amylase for 4 hfollowed by protease for 8 h, and finally by folate conjugase for 3 hsignificantly increased the measurable folate in human milk by an average of 85% compared with values after folate conjugase treatment alone (p < 0.001). Neither the pH of the extraction buffer, the source of folate conjugase, nor the method/length of heat treatment of the samples were significant variables influencing the values of human milk folate. Data indicate that folate in human milk can be underestimated unless samples are treated with cr-amylase, protease, and folate conjugase prior to microbiological assay using L. casei. (C) 1998 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/10/20 alle ore 21:32:28