Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
ALPHA-SNAP BUT NOT GAMMA-SNAP IS REQUIRED FOR ER-GOLGI TRANSPORT AFTER VESICLE BUDDING AND THE RAB1-REQUIRING STEP BUT BEFORE THE EGTA-SENSITIVE STEP
Autore:
PETER F; WONG SH; SUBRAMANIAM VN; TANG BL; HONG WJ;
Indirizzi:
INST MOL & CELL BIOL,MEMBRANE BIOL LAB,30 MED DR SINGAPORE 117609 SINGAPORE INST MOL & CELL BIOL,MEMBRANE BIOL LAB SINGAPORE 117609 SINGAPORE
Titolo Testata:
Journal of Cell Science
, volume: 111, anno: 1998,
parte:, 17
pagine: 2625 - 2633
SICI:
0021-9533(1998)111:<2625:ABNGIR>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
SEMI-INTACT CELLS; ENDOPLASMIC-RETICULUM; PROTEIN-TRANSPORT; BIOCHEMICAL REQUIREMENTS; VESICULAR TRANSPORT; GENE-PRODUCT; RAB PROTEINS; FUSION; YEAST; NSF;
Keywords:
SNAP; VESICULAR TRANSPORT; DOCKING/FUSION; ER; GOLGI;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
48
Recensione:
Indirizzi per estratti:
Citazione:
F. Peter et al., "ALPHA-SNAP BUT NOT GAMMA-SNAP IS REQUIRED FOR ER-GOLGI TRANSPORT AFTER VESICLE BUDDING AND THE RAB1-REQUIRING STEP BUT BEFORE THE EGTA-SENSITIVE STEP", Journal of Cell Science, 111, 1998, pp. 2625-2633

Abstract

N-ethylmaleimide-sensitive factor (NSF) and soluble NSF attachment proteins (SNAPs) have been implicated in diverse vesicular transport events; yet their exact role and site of action remain to be established. Using an established in vitro system, we show that antibodies againstalpha-SNAP inhibit vesicle transport from the ER to the cis-Golgi andthat recombinant alpha-SNAP enhances/stimulates the process. Cytosol immunodepleted of alpha-SNAP does not support normal transport unless supplemented with recombinant alpha-SNAP but not gamma-SNAP. In markedcontrast, cytosol immunodepleted of gamma-SNAP supports ER-Golgi transport to the normal level. Neither antibodies against gamma-SNAP nor recombinant gamma-SNAP have any effect on ER-Golgi transport. These results clearly establish an essential role for alpha-SNAP but not gamma-SNAP in ER-Golgi transport. When the transport assay is performed withcytosol immunodepleted of alpha-SNAP, followed by incubation with cytosol immunodepleted of a COPII subunit, normal transport is achieved. In marked contrast, no transport is detected when the assay is first performed with cytosol depleted of the COPII subunit followed by alpha-SNAP-depleted cytosol, suggesting that alpha-SNAP is required after a step that requires COPII (the budding step). In combination with cytosol immunodepleted of Rab1, it is seen that alpha-SNAP is required after a Rab1-requiring step. It has been shown previously that EGTA blocksER-Golgi transport at a step after vesicle docking but before fusion and we show here that alpha-SNAP acts before the step that is blocked by EGTA, Our results suggest that alpha-SNAP may be involved in the pre-docking or docking but not the fusion process.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 06:48:34