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Titolo:
FACTORS INFLUENCING THE CHOICE OF BUFFER IN BACKGROUND ELECTROLYTES FOR INDIRECT DETECTION OF FAST ANIONS BY CAPILLARY-ELECTROPHORESIS
Autore:
DOBLE P; MACKA M; HADDAD PR;
Indirizzi:
UNIV TASMANIA,DEPT CHEM,GPO BOX 252-75 HOBART TAS 7001 AUSTRALIA UNIV TASMANIA,DEPT CHEM HOBART TAS 7001 AUSTRALIA
Titolo Testata:
Electrophoresis
fascicolo: 12, volume: 19, anno: 1998,
pagine: 2257 - 2261
SICI:
0173-0835(1998)19:12<2257:FITCOB>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
INDIRECT UV DETECTION; INDIRECT PHOTOMETRIC DETECTION; MOLECULAR-MASS ANIONS; ZONE ELECTROPHORESIS; ION ELECTROPHORESIS; INORGANIC ANIONS; BAYER LIQUOR; SEPARATION; OPTIMIZATION; SELECTIVITY;
Keywords:
BACKGROUND ELECTROLYTE; CAPILLARY ELECTROPHORESIS; INDIRECT DETECTION; FAST ANIONS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
32
Recensione:
Indirizzi per estratti:
Citazione:
P. Doble et al., "FACTORS INFLUENCING THE CHOICE OF BUFFER IN BACKGROUND ELECTROLYTES FOR INDIRECT DETECTION OF FAST ANIONS BY CAPILLARY-ELECTROPHORESIS", Electrophoresis, 19(12), 1998, pp. 2257-2261

Abstract

The suitability of relatively slow (low absolute value of mobility) coanionic buffers in background electrolytes (BGEs) for indirect photometric detection of anions by capillary electrophoresis was investigated. As a model system, 2-(cyclohexylamino)ethanesulfonic acid (CHES) was used to buffer the indirect detection electrolyte of sodium chromate. CHES (pK(a) 9.55) is a zwitterionic molecule carrying a net negativecharge depending on the pH (effective charge -0.5 at pH = pK(a)). Within its useful pH buffering range CHES acted as a competing probe coanion. System peaks were induced which had deleterious effects on the detection sensitivity of slow to medium mobility anions. The mobility ofthe system peak was determined by the effective mobility of CHES, both of which increased with increasing pH. The peaks of analytes that migrated near or on the system peak were distorted and lost all quantitative properties. Analytes that migrated after the system peak either were not detected or reversed their responses. Analytes that migrated well before the system peak were unaffected. Consequently, the suitability of slow coanionic buffers is limited either to (i) fast anions or,(ii) a pH range much below the pK(a), where the buffering capacity isnot optimal.

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Documento generato il 07/07/20 alle ore 22:30:39