Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
INFLUENCE OF MITE GROWTH CULTURE PHASES ON THE BIOLOGICAL STANDARDIZATION OF ALLERGENIC EXTRACTS
Autore:
ERASO E; MARTINEZ J; GARCIAORTEGA P; MARTINEZ A; PALACIOS R; CISTERNA R; GUISANTES JA;
Indirizzi:
IFIDESA,R&D DEPT,ALAMEDA URQUIJO 27 BILBAO 48008 SPAIN
Titolo Testata:
Journal of investigational allergology & clinical immunology
fascicolo: 4, volume: 8, anno: 1998,
pagine: 201 - 206
SICI:
1018-9068(1998)8:4<201:IOMGCP>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
DERMATOPHAGOIDES-PTERONYSSINUS; TESTS;
Keywords:
HOUSE DUST MITES; DERMATOPHAGOIDES PTERONYSSINUS; DERMATOPHAGOIDES FARINAE; ALLERGY; BIOLOGICAL STANDARDIZATION; ALLERGENIC EXTRACTS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
20
Recensione:
Indirizzi per estratti:
Citazione:
E. Eraso et al., "INFLUENCE OF MITE GROWTH CULTURE PHASES ON THE BIOLOGICAL STANDARDIZATION OF ALLERGENIC EXTRACTS", Journal of investigational allergology & clinical immunology, 8(4), 1998, pp. 201-206

Abstract

House dust mites are a well known cause of asthma and other respiratory allergies. In order to improve the standardization of allergenic extracts for diagnosis and immunotherapy, it is important to determine the frequency and concentration of the components, both the major and the minor allergens during the growth period of the mite population. Ina previous paper we demonstrated that the laboratory cultures of Dermatophagoides pteronyssinus and Dermatophagoides farinae exhibited three well differentiated growth phases: latency, exponential growth, and death of the culture. Biological standardization of extracts from the two mite species were carried out by skin prick tests in a group of 20patients, using different concentrations of the extracts at the threegrowth phases. The patient sera were also studied by means of the RAST technique to determine the levels of specific IgE for each phase. The extracts produced from the exponential growth phase of the cultures revealed six times more relative allergenic activity in in vivo studies, and average RAST values were approximately three times higher than those extracts from latency and death phases. The reproducibility of the extract production method was assessed by comparing different batches obtained in similar conditions. The results showed batch-to-batch homogeneity allergenic activity. In conclusion, if was demonstrated that extracts obtained from cultures with the highest concentration of live mites (maximum growth phase) render the best diagnostic results in vivo and in vitro.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 07:49:27