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Titolo:
A NOVEL TYPE OF ADHERING JUNCTION IN AN EPITHELIOID TUMORIGENIC RAT-CELL CULTURE LINE
Autore:
SCHMELZ M; WAY DL; BORGS P; PEITSCH WK; SCHMIDT H; WITTE MH; WITTE CL; FRANKE WW; MOLL R;
Indirizzi:
GERMAN CANC RES CTR,DIV CELL BIOL,NEUENHEIMER FELD 280 D-69120 HEIDELBERG GERMANY GERMAN CANC RES CTR,DIV CELL BIOL D-69120 HEIDELBERG GERMANY UNIV HALLE WITTENBERG,DEPT PATHOL D-06097 HALLE GERMANY UNIV ARIZONA,COLL MED,DEPT SURG TUCSON AZ 00000 UNIV GUELPH,DEPT VET MICROBIOL & IMMUNOL GUELPH ON N1G 2W1 CANADA
Titolo Testata:
Cell and tissue research
fascicolo: 1, volume: 294, anno: 1998,
pagine: 11 - 25
SICI:
0302-766X(1998)294:1<11:ANTOAJ>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTERMEDIATE FILAMENT ANCHORAGE; DESMOSOMAL PLAQUE PROTEINS; AMINO-ACID-SEQUENCE; CONSTITUTIVE TRANSMEMBRANE GLYCOPROTEIN; DESMOPLAKIN-CONTAINING JUNCTIONS; UVOMORULIN-CATENIN COMPLEX; NON-EPIDERMAL DESMOSOMES; HUMAN-ENDOTHELIAL-CELLS; ADHESION MOLECULES; CADHERIN FAMILY;
Keywords:
ADHERING JUNCTIONS; DESMOSOMES; ENDOTHELIAL JUNCTIONS; PLAQUE PROTEINS; DESMOPLAKIN; CADHERINS; PROTEIN ZO-1; RAT, CELL CULTURE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
97
Recensione:
Indirizzi per estratti:
Citazione:
M. Schmelz et al., "A NOVEL TYPE OF ADHERING JUNCTION IN AN EPITHELIOID TUMORIGENIC RAT-CELL CULTURE LINE", Cell and tissue research, 294(1), 1998, pp. 11-25

Abstract

Two major types of plaque-bearing adhering junctions are commonly distinguished: the actin microfilament-anchoring adhaerens junctions (AJs) and the desmosomes anchoring intermediate-sized filaments (IFs). Both types of junction usually possess the common plaque protein, plakoglobin, whereas the other plaque proteins and the transmembrane cadherins are mutually exclusive. For example, AJs contain E-, N-, or P-cadherin in combination with alpha- and beta-catenin, vinculin and alpha-actinin, whereas in desmosomes, desmogleins and desmocollins are associated with desmoplakin and one or several of the plakophilins (PP1-3). Here we describe a novel type of adhering junction comprising proteins of both AJs and desmosomes and the tight junction (TJ) plaque protein, ZO-1, in a newly established, liver-derived tumorigenic rat cell line (RMEC-1). By immunofluorescence microscopy, cell-cell contacts are characterized by mostly continuous-appearing lines which are usually resolved by electron microscopy as extended arrays of closely spaced smallplaque subunits. These plaque-covered regions are positive for plakoglobin, alpha- and beta-catenin, the arm-repeat protein p120, vinculin,desmoplakin and protein ZO-1. They are positive for E-cadherin in cultures early on in passaging, but tend to turn negative for all known cadherins in densely grown cultures. On immunoblotting SDS-PAGE-separated proteins from dense-grown cell monolayers, ''pan-cadherin'' antibodies have reacted with a band at similar to 140 kDa, identified as N-cadherin by peptide fingerprinting of the immunoprecipitated protein, which for reasons not yet clear is modified or masked in immunolocalization experiments. The exact histological derivation of RMEC-1 cells is not known. However, the observations of several endothelial markers and the fact that all cells are rich in IFs containing vimentin and/or desmin, while only subpopulations also reveal Ifs containing CKs 8 and 18, is suggestive of a mesenchymal, probably endothelial origin. We discuss the molecular relationship of this novel type of extended junction with other types of adhering junctions.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/07/20 alle ore 07:24:53