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Titolo:
GLUCOSE STIMULATION OF PANCREATIC BETA-CELL LINES INDUCES EXPRESSION AND SECRETION OF DYNORPHIN
Autore:
JOSEFSEN K; BUSCHARD K; SORENSEN LR; WOLLIKE M; EKMAN R; BIRKENBACH M;
Indirizzi:
KOMMUNE HOSP COPENHAGEN,BARTHOLIN INST DK-1399 COPENHAGEN K DENMARK UNIV GOTHENBURG,MOLNDAL HOSP,DEPT CLIN NEUROSCI S-43180 GOTHENBURG SWEDEN UNIV CHICAGO,KOVLER VIRAL ONCOL LAB CHICAGO IL 60637
Titolo Testata:
Endocrinology
fascicolo: 10, volume: 139, anno: 1998,
pagine: 4329 - 4336
SICI:
0013-7227(1998)139:10<4329:GSOPBL>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENDOGENOUS OPIOID-PEPTIDES; RAT PRODYNORPHIN GENE; INSULIN-SECRETION; INCREASED SENSITIVITY; TRANSCRIPTION FACTOR; ENDOCRINE PANCREAS; GUINEA-PIG; ISLETS; LANGERHANS; PROTEIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
65
Recensione:
Indirizzi per estratti:
Citazione:
K. Josefsen et al., "GLUCOSE STIMULATION OF PANCREATIC BETA-CELL LINES INDUCES EXPRESSION AND SECRETION OF DYNORPHIN", Endocrinology, 139(10), 1998, pp. 4329-4336

Abstract

To investigate adaptive responses of pancreatic beta-cells to hyperglycemia, genes induced by glucose stimulation were identified by subtraction cloning. Among 53 clones representing differentially expressed genes, 20 encoded the endogenous opioid precursor, prodynorphin. The amino acid sequence of murine prodynorphin is identical to the rat protein in sequences comprising the opioid peptides and 86% identical in the remainder of the molecule. Stimulation of MIN6 cells increased prodynorphin RNA levels to more than 20-fold in proportion to physiologicalglucose concentrations. Similar induction levels were observed in murine beta TC3 and rat Rinm5F beta-cell lines. Prodynorphin RNA expression increased within 1 h of glucose stimulation, achieved maximal levels by 4 h, and remained elevated for at least 24 h. By using RIA, MING cells were shown to contain and secrete increased amounts of dynorphin-ll following glucose stimulation. Treatment of MING cells with KCl, forskolin, or isobutyl-methyl-xanthine strongly induced prodynorphin RNA expression, suggesting that induction may be related to secretion-coupled signaling pathways. The induction of prodynorphin in several beta-cell lines is consistent with previous demonstrations of beta-cell synthesis of other endogenous opioids, including beta-endorphin, and suggests that opioids may have a potentially significant role in regulating beta-cell secretion.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 22:06:54