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Titolo:
IN-VITRO MORPHOLOGICAL AND FUNCTIONAL-CHARACTERIZATION OF ISOLATED PORCINE HEPATOCYTES FOR EXTRACORPOREAL LIVER SUPPORT - BILE-ACID UPTAKE AND CONJUGATION
Autore:
MORSIANI E; PAZZI P; MOSCIONI AD; ROZGA J; AZZENA G; DEMETRIOU AA;
Indirizzi:
UNIV CALIF LOS ANGELES,CEDARS SINAI MED CTR,DEPT SURG LOS ANGELES CA 90048 UNIV FERRARA,SCH MED,DEPT SURG I-44100 FERRARA ITALY UNIV FERRARA,SCH MED,DEPT GASTROENTEROL I-44100 FERRARA ITALY
Titolo Testata:
The Journal of surgical research (Print)
fascicolo: 1, volume: 79, anno: 1998,
pagine: 54 - 60
SICI:
0022-4804(1998)79:1<54:IMAFOI>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
MICROCARRIER-ATTACHED HEPATOCYTES; FULMINANT HEPATIC-FAILURE; BIOARTIFICIAL LIVER; EXTRACELLULAR-MATRIX; RAT HEPATOCYTES; SHORT-TERM; TRANSPLANTATION; CULTURE;
Keywords:
PORCINE HEPATOCYTES; BILE ACID METABOLISM; BIOARTIFICIAL LIVER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
35
Recensione:
Indirizzi per estratti:
Citazione:
E. Morsiani et al., "IN-VITRO MORPHOLOGICAL AND FUNCTIONAL-CHARACTERIZATION OF ISOLATED PORCINE HEPATOCYTES FOR EXTRACORPOREAL LIVER SUPPORT - BILE-ACID UPTAKE AND CONJUGATION", The Journal of surgical research (Print), 79(1), 1998, pp. 54-60

Abstract

Recently, researchers have focused on the use of bioartificial liver (BAL) to support patients with fulminant hepatic failure (FHF). We have developed a cell-based BAL, consisting of porcine hepatocytes in a hollow-fiber bioreactor. To better characterize BAL metabolic functionsin vitro, bioreactors were inoculated with 48-h-cultured, microcarrier-attached hepatocytes and perifused with recirculating human plasma that contained either 1 mu ci of [24-C-14] plasma-enriched cholate or 1mu Ci of [24-C-14] plasma-enriched taurocholate. Bile acids were sampled hourly and separated into four fractions (unconjugated, glycoconjugated, tauroconjugated, and sulfated) for radioactivity determination. Following 3 h perifusion, the glycoconjugated and sulfated bile acid fractions in the bioreactor extrafiber space were significantly elevated when compared to the recirculating plasma. During perifusion with taurocholate-enriched plasma, a relative decrease in the tauroconjugated fraction and an increase in the glycoconjugated fr-action were observed. Cholate was accumulated by hepatocytes to a level threefold lowerthan taurocholate; however, a significant proportion of radioactivity(<25%) was detected in the glycoconjugated fraction. Ultrastructural examination of microcarrier-attached hepatocytes illustrated that the features typical of metabolically active liver cells were maintained, Our data demonstrate the ability of BAL to clear bile acids from the circulation, to accumulate cholate and taurocholate, and to conjugate asubstantial amount of cholic acid, (C) 1998 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/21 alle ore 14:36:30