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Titolo:
SPECIFIC DETECTION OF INTRACELLULAR SYMBIOTIC BACTERIA OF APHIDS BY OLIGONUCLEOTIDE-PROBED IN-SITU HYBRIDIZATION
Autore:
FUKATSU T; WATANABE K; SEKIGUCHI Y;
Indirizzi:
NATL INST BIOSCI & HUMAN TECHNOL,AGCY IND SCI & TECHNOL,1-1 HIGASHI IBARAKI OSAKA 3058566 JAPAN NAGAOKA UNIV TECHNOL,DEPT ENVIRONM SYST ENGN NIIGATA 9402188 JAPAN
Titolo Testata:
Applied Entomology and Zoology
fascicolo: 3, volume: 33, anno: 1998,
pagine: 461 - 472
SICI:
0003-6862(1998)33:3<461:SDOISB>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACYRTHOSIPHON-PISUM; MICROORGANISMS; EVOLUTION; PHYLOGENY; IDENTIFICATION; ENDOSYMBIONTS; CELLS; DNA; GUT;
Keywords:
APHID; INTRACELLULAR SYMBIOTIC BACTERIA; PRIMARY AND SECONDARY SYMBIONTS; IN SITU HYBRIDIZATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
31
Recensione:
Indirizzi per estratti:
Citazione:
T. Fukatsu et al., "SPECIFIC DETECTION OF INTRACELLULAR SYMBIOTIC BACTERIA OF APHIDS BY OLIGONUCLEOTIDE-PROBED IN-SITU HYBRIDIZATION", Applied Entomology and Zoology, 33(3), 1998, pp. 461-472

Abstract

Oligonucleotide-probed in situ hybridization targeted to bacterial rRNAs was attempted to detect, visualize and characterize the intracellular symbiotic bacteria of aphids. Using the EUB338 probe that hybridizes universally with 16S rRNA of eubacteria, the primary and secondary intracellular symbionts of various aphids were successfully stained ontissue thin sections. When in situ hybridization was conducted with GAM42A and BET42A probes that are targeted to 23S rRNA of gamma- and P-subdivision of the Proteobacteria, respectively, it was shown that thesecondary symbionts of Cinara pini and Nippolachnus piri belong to the gamma-subdivision of the Proteobacteria whereas that of Tetraneura radicicola is a member of the beta-subdivision of the Proteobacteria. This is the first report on the phylogenetic affinity of the secondary intracellular symbionts of aphids. Because insect tissues have strong autofluorescence in general, non-fluorescent in situ hybridization using biotin- and digoxigenin-labelled probes was more suitable for detecting symbiotic bacteria in aphid tissue sections.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 13:44:08