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Titolo:
SYSTEMATIC-APPROACH TO METHODS DEVELOPMENT FOR THE CAPILLARY ELECTROPHORETIC ANALYSIS OF A MINOR ENANTIOMER USING A SINGLE-ISOMER SULFATED CYCLODEXTRIN - A CASE-STUDY OF L-CARBIDOPA ANALYSIS
Autore:
VINCENT JB; VIGH G;
Indirizzi:
TEXAS A&M UNIV,DEPT CHEM COLLEGE STN TX 77842 TEXAS A&M UNIV,DEPT CHEM COLLEGE STN TX 77842
Titolo Testata:
Journal of chromatography
fascicolo: 1-2, volume: 817, anno: 1998,
pagine: 105 - 111
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHIRAL RESOLVING AGENTS; ZONE ELECTROPHORESIS; MOBILITY; FAMILY;
Keywords:
METHOD DEVELOPMENT; ENANTIOMER SEPARATION; BACKGROUND ELECTROLYTE COMPOSITION; CYCLODEXTRINS; HEPTAKIS(2,3-DIACETYL-6-SULFATO)-BETA-CYCLODEXTRIN; CARBIDOPA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
22
Recensione:
Indirizzi per estratti:
Citazione:
J.B. Vincent e G. Vigh, "SYSTEMATIC-APPROACH TO METHODS DEVELOPMENT FOR THE CAPILLARY ELECTROPHORETIC ANALYSIS OF A MINOR ENANTIOMER USING A SINGLE-ISOMER SULFATED CYCLODEXTRIN - A CASE-STUDY OF L-CARBIDOPA ANALYSIS", Journal of chromatography, 817(1-2), 1998, pp. 105-111

Abstract

A methods development protocol - based on the charged resolving agentmigration model - for the capillary electrophoretic analysis of a minor enantiomer using a single-isomer sulfated cyclodextrin is describedhere. The minor component of L-carbidopa was successfully assayed by capillary electrophoresis using the single-isomer eptakis-(2,3-diacetyl-6-sulfato)-beta-cyclodextrin as chiral resolving agent. Adequate separation selectivity was secured by selecting a pH 2.5 background electrolyte, operating at 16 degrees C and adjusting the concentration of the single-isomer sulfated cyclodextrin to just past the point where the migration direction of both enantiomers of carbidopa changes from cationic to anionic due to complexation with the single-isomer sulfated cyclodextrin. Once the background electrolyte composition which leads to adequate selectivity was identified, peak resolution was adjusted by optimizing the magnitude of the dimensionless normalized electroosmotic flow-rate via the addition of poly(ethylene glycol)900 which does not change separation selectivity. Rugged separation conditions permitting the fast, reproducible quantitation of the minor enantiomer in L-carbidopa preparations could be readily established. (C) 1998 ElsevierScience B.V. All rights reserved.

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Documento generato il 24/09/20 alle ore 11:24:38