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Titolo:
PRODOMAIN-DEPENDENT NUCLEAR-LOCALIZATION OF THE CASPASE-2 (NEDD2) PRECURSOR - A NOVEL FUNCTION FOR A CASPASE PRODOMAIN
Autore:
COLUSSI PA; HARVEY NL; KUMAR S;
Indirizzi:
INST MED & VET SCI,HANSON CTR CANC RES,POB 14,RUNDLE MALL,FROME RD ADELAIDE SA 5000 AUSTRALIA INST MED & VET SCI,HANSON CTR CANC RES ADELAIDE SA 5000 AUSTRALIA
Titolo Testata:
The Journal of biological chemistry
fascicolo: 38, volume: 273, anno: 1998,
pagine: 24535 - 24542
SICI:
0021-9258(1998)273:38<24535:PNOTC(>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
ICE-LIKE PROTEASES; CELL-DEATH; CYSTEINE PROTEASE; GRANZYME-B; APOPTOSIS; ENZYME; EXPRESSION; FAMILY; CED-3; CPP32;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
P.A. Colussi et al., "PRODOMAIN-DEPENDENT NUCLEAR-LOCALIZATION OF THE CASPASE-2 (NEDD2) PRECURSOR - A NOVEL FUNCTION FOR A CASPASE PRODOMAIN", The Journal of biological chemistry, 273(38), 1998, pp. 24535-24542

Abstract

Caspases are cysteine proteases that play an essential role in apoptosis by cleaving several key cellular proteins. Despite their function in apoptosis, little is known about where in the cell they are localized and whether they are translocated to specific cellular compartmentsupon activation. In the present paper, using Aequorea victoria green fluorescent protein fusion constructs, we have determined the localization of Nedd2 (mouse caspase-2) and show that both precursor and processed caspase-2 localize to the cytoplasmic and the nuclear compartments. We demonstrate that the nuclear localization of caspase-2 is strictly dependent on the presence of the prodomain. A caspase-2 prodomain-green fluorescent protein localized to dot- and fiber-like structures mostly in the nucleus, whereas a protein lacking the prodomain was largely concentrated in the cytoplasm, We also show that an amino-terminalfusion of the prodomain of caspase-2 to caspase-3 mediates nuclear transport of caspase-3, which is normally localized in the cytoplasm. These results suggest that, in addition to roles in dimerization and recruitment through adaptors, the caspase-2 prodomain has a novel function in nuclear transport.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 22:03:14