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Titolo:
METASTATIC CUTANEOUS MELANOMA PROMOTED BY ULTRAVIOLET-RADIATION IN MICE WITH TRANSGENE-INITIATED LOW MELANOMA SUSCEPTIBILITY
Autore:
KELSALL SR; MINTZ B;
Indirizzi:
FOX CHASE CANC CTR,INST CANC RES,7701 BURHOLME AVE PHILADELPHIA PA 19111 FOX CHASE CANC CTR,INST CANC RES PHILADELPHIA PA 19111
Titolo Testata:
Cancer research
fascicolo: 18, volume: 58, anno: 1998,
pagine: 4061 - 4065
SICI:
0008-5472(1998)58:18<4061:MCMPBU>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
MALIGNANT SKIN MELANOMA; MURINE MELANOMAS; MOUSE MODEL; DNA-DAMAGE; GROWTH; MELANOGENESIS; EXPRESSION; TYROSINASE; INDUCTION; REPAIR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
S.R. Kelsall e B. Mintz, "METASTATIC CUTANEOUS MELANOMA PROMOTED BY ULTRAVIOLET-RADIATION IN MICE WITH TRANSGENE-INITIATED LOW MELANOMA SUSCEPTIBILITY", Cancer research, 58(18), 1998, pp. 4061-4065

Abstract

An inbred-strain (C57BL/6) transgenic (Tyr-SV40E) mouse model of ultraviolet radiation (UVR)-induced metastatic cutaneous melanoma was produced without the use of chemical carcinogens and without resulting in other skin malignancies. Expression of this transgene occurs specifically in melanocytic-lineage cells. In untreated hemizygous mice of transgenic line 12 there are no skin melanomas, and the oncogenic sequence, which is expressed at a very low level, functions solely as a weak initiating stimulus. UVR [including 65% ultraviolet B (280-320 nm wavelength)] supplied the necessary promoting stimulus leading to melanomas. Of various trial protocols, eight were successful and involved exposure of 112 mice for a limited time on each of 3-10 days starting at 2-3 days of age and totalling 1.13.7 J/cm(2) UVR. Fourteen of these animals developed a total of 15 invasive skin melanomas on the head and body, arising between 37-115 weeks of age and, therefore, often after a relatively long latency. The tumors were melanotic and in five of the mice they yielded macrometastases in regional and distant sites. The single most favorable protocol (1.9 J/cm(2) total UVR, at 0.38 J/cm(2)/day for 5 days starting at 3 days of age) led to the highest incidenceof melanoma (5 of 19 mice) and one of the lowest mortality rates (2 of 19). No melanomas occurred in UVR-treated nontransgenic C57BL/6 controls. Benign skin keratoacanthomas arose and often regressed in treated transgenic as well as nontransgenic mice. This new transgenic mouse model introduces many novel possibilities for experimental analysis ofthe melanoma-promoting mechanisms of UVR and also of the ability of specific genetic changes to impede or facilitate the UVR effect.

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Documento generato il 28/11/20 alle ore 09:52:16