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Titolo:
LI-7 NUCLEAR-MAGNETIC-RESONANCE STUDY FOR THE DETERMINATION OF LI-SY5Y CELLS( PROPERTIES IN NEUROBLASTOMA SH)
Autore:
NIKOLAKOPOULOS J; ZACHARIAH C; DEFREITAS DM; STUBBS EB; RAMASAMY R; CASTRO GMCA; GERALDES CFGC;
Indirizzi:
LOYOLA UNIV,DEPT CHEM,6525 N SHERIDAN RD CHICAGO IL 60626 LOYOLA UNIV,DEPT CHEM CHICAGO IL 60626 US DEPT VET AFFAIRS,VET AFFAIRS EDWARD HINES JR HOSP,SERV NEUROL HINES IL 60141 LOYOLA UNIV,MED CTR,DEPT NEUROL MAYWOOD IL 60153 LOYOLA UNIV,MED CTR,DEPT MOL & CELLULAR BIOCHEM MAYWOOD IL 60153 COLUMBIA UNIV COLL PHYS & SURG,DIV CARDIOL NEW YORK NY 00000 UNIV COIMBRA,DEPT BIOCHEM COIMBRA PORTUGAL UNIV COIMBRA,CTR NEUROSCI COIMBRA PORTUGAL
Titolo Testata:
Journal of neurochemistry
fascicolo: 4, volume: 71, anno: 1998,
pagine: 1676 - 1684
SICI:
0022-3042(1998)71:4<1676:LNSFTD>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
RED-BLOOD-CELLS; HUMAN-ERYTHROCYTES; NMR-SPECTROSCOPY; SHIFT-REAGENT; GEL THREADS; TRANSPORT; BINDING; EXPRESSION; MEMBRANE; AGONISTS;
Keywords:
PERFUSION; AGAROSE GEL THREADS; SHIFT REAGENTS; VERATRIDINE; NA+ CHANNELS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
J. Nikolakopoulos et al., "LI-7 NUCLEAR-MAGNETIC-RESONANCE STUDY FOR THE DETERMINATION OF LI-SY5Y CELLS( PROPERTIES IN NEUROBLASTOMA SH)", Journal of neurochemistry, 71(4), 1998, pp. 1676-1684

Abstract

Lithium has been used clinically in the treatment of manic depression, However, its pharmacologic mode of action remains unclear, Characteristics of Li+ interactions in red blood cells (RBCs) have been identified. We investigated Li+ interactions on human neuroblastoma SH-SY5Y cells by developing a novel Li-7 NMR method that provided a clear estimation of the intra- and extracellular amounts of Li+ in the presence of the shift reagent thulium-1,4,7,10-tetrazacyclododecane-N, N',N '', N'''-tetramethylene phosphonate (HTmDOTP4-). The first-order rate constants of Li+ influx and efflux for perfused, agarose-embedded SH-SY5Y cells in the presence of 3 mM HTmDOTP4- were 0.055 +/- 0.006 (n = 4) and -0.025 +/- 0.006 min(-1) (n = 3), respectively, Significant increases in the rate constants of Li+ influx and efflux in the presence of 0.05 mM veratridine indicated the presence of Nai channel-mediated Li+ transport in SH-SY5Y cells. Li-7 NMR relaxation measurements showed that Li+ is immobilized more in human neuroblastoma SH-SY5Y cells than in human RBCs.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 09:25:14