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Titolo:
ATP RELEASED BY LPS INCREASES NITRIC-OXIDE PRODUCTION IN RAW-264.7 MACROPHAGE CELL-LINE VIA P2Z P2X7 RECEPTORS/
Autore:
SPERLAGH B; HASKO G; NEMETH Z; VIZI ES;
Indirizzi:
HUNGARIAN ACAD SCI,INST EXPT MED,DEPT PHARMACOL,POB 67 BUDAPEST HUNGARY
Titolo Testata:
Neurochemistry international
fascicolo: 3, volume: 33, anno: 1998,
pagine: 209 - 215
SICI:
0197-0186(1998)33:3<209:ARBLIN>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
RAW-264.7 MURINE MACROPHAGES; PURINERGIC P2Z RECEPTOR; EXTRACELLULAR ATP; MICROGLIAL CELLS; SYNTHASE EXPRESSION; HUMAN-LYMPHOCYTES; P2X(7) RECEPTOR; NERVOUS-SYSTEM; TNF-ALPHA; PURINOCEPTOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
45
Recensione:
Indirizzi per estratti:
Citazione:
B. Sperlagh et al., "ATP RELEASED BY LPS INCREASES NITRIC-OXIDE PRODUCTION IN RAW-264.7 MACROPHAGE CELL-LINE VIA P2Z P2X7 RECEPTORS/", Neurochemistry international, 33(3), 1998, pp. 209-215

Abstract

P2Z/P2X7 receptor is a particular type of purinoceptor. which is selectively expressed on the surface of immune cells in neuronal and non-neuronal tissues. Despite intensive research on its involvement in the immune response, the exact mechanism whereby it affects intercellular signaling is far from clear yet. In this study, the effect of activation P2Z/P2X7 receptor was investigated on the bacterial lipopolysaccharide induced nitric oxide production in RAW 264.7 macrophage call line using the nitrite/nitrate assay. The P2Z/P2X7 receptor agonist 3'-O-(4-benzoylbenzoyl)-adenosine 5'triphosphate increased concentration-dependency the lipoplysaccharide(10 mu g/ml) induced nitric oxide production between 10 mu M and 250 mu M. ATP also increased nitric oxide production in response to lipopolysaccharide, while ADP, 2-methylo-thio-adenosine 5'-triphosphate and adenosine 5'triphosphate-gamma-S was without effect. Pretreatment with oxidized adenosine triphosphate, the selective P2Z/P2X7 receptor antagonise (300 mu M-1 mu M) strongly decreasedlipopolysaccaride induced nitric oxide production. Furthermore, on macrophages, pretreated with oxidized adenosine 5'triphosphate (300 mu M- 1 mM), 3'-O-(4-benzoylbenzoyl)-adenosine 5'-triphosphate and ATP did not affect lipopolysaccharide induced nitric oxide production. 15 min lipopolysaccharide treatment induced a transient and reversible release of endogenous ATP from RAW 264.7 cells, measured by the luciferin-luciferase assay. The effect of lipopolysaccharide to promote ATP release was concentration-dependent between 1-10 mu g/ml. In summary, our results show that P2Z/P2X7 receptor activation results in an increase in nitric oxide production in response to lipopolysaccharide challenge. Since the P2Z/P2X7 receptor antagonist oxidized adenosine triphosphate decreased lipopolysaccharide induced nitric oxide production, and lipopolysaccharide was able to promote ATP release from macrophage cells, it seems likely that endogenous ATP is involved in nitric oxide formation during endotoxin challenge. (C) 1998 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 18:39:18