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Titolo:
CHARACTERIZATION OF LEMIR, A ROOT-KNOT NEMATODE-INDUCED GENE IN TOMATO WITH AN ENCODED PRODUCT SECRETED FROM THE ROOT
Autore:
BRENNER ED; LAMBERT KN; KALOSHIAN I; WILLIAMSON VM;
Indirizzi:
UNIV CALIF DAVIS,DEPT NEMATOL LIVERMORE CA 95616 UNIV CALIF DAVIS,DEPT NEMATOL LIVERMORE CA 95616
Titolo Testata:
Plant physiology
fascicolo: 1, volume: 118, anno: 1998,
pagine: 237 - 247
SICI:
0032-0889(1998)118:1<237:COLARN>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
TASTE-MODIFYING PROTEIN; AMINO-ACID-SEQUENCE; PATHOGENESIS-RELATED PROTEINS; PLANT ANTIFUNGAL PROTEINS; DEFENSE-RELATED PROTEINS; ALPHA-AMYLASE; DIFFERENTIAL EXPRESSION; LYCOPERSICON-ESCULENTUM; TRICHOSANTHES KIRILOWII; MELOIDOGYNE-INCOGNITA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
69
Recensione:
Indirizzi per estratti:
Citazione:
E.D. Brenner et al., "CHARACTERIZATION OF LEMIR, A ROOT-KNOT NEMATODE-INDUCED GENE IN TOMATO WITH AN ENCODED PRODUCT SECRETED FROM THE ROOT", Plant physiology, 118(1), 1998, pp. 237-247

Abstract

A tomato gene that is induced early after infection of tomato (Lycopersicon esculentum Mill.) with root-knot nematodes (Meloidogyne javanica) encodes a protein with 54% amino acid identity to miraculin, a flavorless protein that causes sour substances to be perceived as sweet. This gene was therefore named LeMir (L. esculentum miraculin). Sequencesimilarity places the encoded protein in the soybean trypsin-inhibitor family (Kunitz). LeMir mRNA is found in root, hypocotyl, and flower tissues, with the highest expression in the root. Rapid induction of expression upon nematode infection is localized to root tips. In situ hybridization shows that LeMir is expressed constitutively in the root-cap and root-tip epidermis. The LeMir protein product (LeMir) was produced in the yeast Pichia pastoris for generation of antibodies. Western-blot analysis showed that LeMir expression is up-regulated by nematode infection and by wounding. LeMir is also expressed in tomato callustissue. Immunoprint analysis revealed that LeMir is expressed throughout the seedling root, but that levels are highest at the root/shoot junction. Analysis of seedling root exudates revealed that LeMir is secreted from the root into the surrounding environment, suggesting that it may interact with soil-borne microorganisms.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/07/20 alle ore 07:53:27