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Titolo:
GROWTH HORMONE-RELEASING PEPTIDE-2 (GHRP-2) DOES NOT ACT VIA THE HUMAN GROWTH HORMONE-RELEASING FACTOR-RECEPTOR IN GC CELLS
Autore:
CHEN C; FARNWORTH P; PETERSENN S; MUSGRAVE I; CANNY BJ; CLARKE IJ;
Indirizzi:
PRINCE HENRYS INST MED RES,POB 5152 CLAYTON VIC 3168 AUSTRALIA MONASH UNIV,DEPT PHYSIOL CLAYTON VIC 3168 AUSTRALIA HAMBERG UNIV,INST HORMONE & FERTIL RES HAMBURG GERMANY
Titolo Testata:
ENDOCRINE
fascicolo: 1, volume: 9, anno: 1998,
pagine: 71 - 77
SICI:
1355-008X(1998)9:1<71:GHP
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-KINASE-C; CULTURED OVINE SOMATOTROPHS; ANTERIOR-PITUITARY CELLS; ADENOSINE-3',5'-MONOPHOSPHATE PRODUCTION; TRANSDUCTION PATHWAYS; RAT SOMATOTROPHS; GH SECRETION; STIMULATION; HEXAPEPTIDE; MECHANISMS;
Keywords:
CAMP; [CA2+]I; SECRETION; PITUITARY; INCUBATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
23
Recensione:
Indirizzi per estratti:
Citazione:
C. Chen et al., "GROWTH HORMONE-RELEASING PEPTIDE-2 (GHRP-2) DOES NOT ACT VIA THE HUMAN GROWTH HORMONE-RELEASING FACTOR-RECEPTOR IN GC CELLS", ENDOCRINE, 9(1), 1998, pp. 71-77

Abstract

Effect of growth hormone-releasing peptide-2 (GHRP-2) on ovine somatotrophs is abolished by a growth hormone-releasing factor (CRF) receptor antagonist, which raises the possibility that GHRP-2 may act on GRF receptors. In the present study, we used rat pituitary CC cells with or without stable transfection of cDNA coding for the human GRF receptor (GC/R+ or GC/R-) to determine whether or not GHRP-2 acts via the CRFreceptor. Northern blot analysis indicated that CRF receptor mRNA wasundetectable in GC/R- cells, whereas a high level of expression occurred in GC/R+ cells that were transfected by CRF receptor cDNA. In GC/R- cells, incubation with up to 10(-7)M of either hGRF or GHRP-2 did not alter the intracellular cAMP, [Ca2+]i, or GH secretion. In GC/R+ cells, hGRF (10(-11)-10(-7)M) increased cAMP levels in a concentration-dependent manner up to 20-fold. This increase in cAMP levels was blockedby a CRF receptor antagonist, [Ac-Tyr(1), D-Arg(2)]-GRF 1-29, but notby a Ca2+ channel blocker, NiCl2 (0.5 mM), GH secretion and [Ca2+]i were, however, not increased by hGRF. Incubation of the transfected cells with 10(-11)-10(-8)M GHRP-2 did not modify intracellular cAMP levels. This result suggests that GHRP-2 does not act through the CRF receptor.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/09/20 alle ore 09:42:25