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Titolo:
FUNCTIONAL AND MOLECULAR CHARACTERIZATION OF LUMINAL AND BASOLATERAL CL- HCO3-, EXCHANGERS OF RAT THICK LIMBS/
Autore:
ELADARI D; BLANCHARD A; LEVIEL F; PAILLARD M; STUARTTILLEY AK; ALPER SL; PODEVIN RA;
Indirizzi:
INST BIOMED CORDELIERS,15 RUE ECOLE MED F-75270 PARIS 06 FRANCE FAC MED,BROUSSAIS HOTEL DIEU,INSERM,UNITE 356,LAB PHYSIOL & ENDOCRINOL CELLULAIRE RENALE PARIS FRANCE HARVARD UNIV,SCH MED,DEPT CELL BIOL BOSTON MA 02215 HARVARD UNIV,SCH MED,DEPT MED BOSTON MA 02215 BETH ISRAEL HOSP,MOL MED UNIT BOSTON MA 02215 BETH ISRAEL HOSP,RENAL UNIT BOSTON MA 02215
Titolo Testata:
American journal of physiology. Renal, fluid and electrolyte physiology
fascicolo: 3, volume: 44, anno: 1998,
pagine: 334 - 342
SICI:
0363-6127(1998)44:3<334:FAMCOL>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
MEMBRANE NA+/H+ EXCHANGE; AE1 ANION-EXCHANGER; ASCENDING LIMB; APICAL MEMBRANE; METABOLIC-ACIDOSIS; CL--HCO3 EXCHANGE; BRUSH-BORDER; H+; PH; HENLE;
Keywords:
ANION EXCHANGE; AE2; BAND 3; AE1; 4,4'-DIISOTHIOCYANOSTILBENE-2,2'-DISULFONIC ACID; MEMBRANE VESICLES; IMMUNOBLOT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
37
Recensione:
Indirizzi per estratti:
Citazione:
D. Eladari et al., "FUNCTIONAL AND MOLECULAR CHARACTERIZATION OF LUMINAL AND BASOLATERAL CL- HCO3-, EXCHANGERS OF RAT THICK LIMBS/", American journal of physiology. Renal, fluid and electrolyte physiology, 44(3), 1998, pp. 334-342

Abstract

Cl-/HCO3- exchange was measured in luminal (LMV) and basolateral (BLMV) membrane vesicles purified from rat medullary thick ascending limb (MTAL). Cl-/HCO3- exchange in BLMV and LMV was inhibited by DIDS, withrespective IC50 values of 3.2 +/- 0.9 and 15.2 +/- 5.2 mu M, whereas Cl- conductances were DIDS insensitive. At constant external pH, BLMV Cl-36(-)/HCO3- and Cl-36(-)/Cl- exchanges exhibited a sigmoidal pattern of activation as internal pH (pH(i)) increased from 6.1 to 8.0, whereas LMV Cl-36(-)/Cl- exchange was unchanged between pH(i) 6.7 and 7.8. The 165-kDa AE2 polypeptide and similar to 115-kDa AE1-related polypeptide were present only in BLMV. In contrast, AE1-related polypeptidesof similar to 90 and 95 kDa were present not only in BLMV but also (in variable abundance) in LMV. mie conclude that rat MTAL BLMV and LMV express distinct anion exchange activities and distinct sets of AE polypeptides. AE2 (and perhaps AE1) in BLMV likely contribute to HCO3- absorption. In contrast, LMV exchangers may contribute to NaCl absorption via parallel coupling with the luminal Na+/H+ antiporters and/or mayprovide negative feedback regulation of HCO3- absorption.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/07/20 alle ore 03:06:02