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Titolo:
INFLUENCE OF ASSAY METHODOLOGY ON THE MEASUREMENT OF FREE SERUM CEFTRIAXONE CONCENTRATIONS
Autore:
KOHLHEPP S; GILBERT DN; LEGGETT JE;
Indirizzi:
PROVIDENCE PORTLAND MED CTR,EARLE A CHILES RES INST,4805 NE GLISAN PORTLAND OR 97213 OREGON HLTH SCI UNIV PORTLAND OR 97201
Titolo Testata:
Antimicrobial agents and chemotherapy
fascicolo: 9, volume: 42, anno: 1998,
pagine: 2259 - 2261
SICI:
0066-4804(1998)42:9<2259:IOAMOT>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLASMA-PROTEIN BINDING; PHARMACOKINETICS; CEPHALOSPORINS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
14
Recensione:
Indirizzi per estratti:
Citazione:
S. Kohlhepp et al., "INFLUENCE OF ASSAY METHODOLOGY ON THE MEASUREMENT OF FREE SERUM CEFTRIAXONE CONCENTRATIONS", Antimicrobial agents and chemotherapy, 42(9), 1998, pp. 2259-2261

Abstract

The influence of assay methodology on the measurement of the active free fraction of ceftriaxone in plasma was determined. The free fraction was measured by three methods: agar diffusion bioassay, precipitation of plasma protein with methanol followed by high-performance liquid chromatography (HPLC) of the supernatant, and ultrafiltration of plasma followed by HPLC of the filtrate. In human serum, the free ceftriaxone levels were significantly lower (P = 0.03) when measured on ultrafiltrates compared to the other two methods. This difference disappearedwhen dolphin serum was studied. After ultrafiltration, human serum was shown, by Scatchard plot analysis, to have two ceftriaxone binding sites. Species differences were also demonstrated. Hence, in humans, determination of free plasma ceftriaxone varies with the assay method employed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/04/20 alle ore 06:59:00